A versatile protein microarray platform enabling antibody profiling against denatured proteins
Article first published online: 17 MAY 2013
© 2012 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim
PROTEOMICS - Clinical Applications
Special Issue: Focus on Cancer Proteomics
Volume 7, Issue 5-6, pages 378–383, June 2013
How to Cite
Wang, J., Barker, K., Steel, J., Park, J., Saul, J., Festa, F., Wallstrom, G., Yu, X., Bian, X., Anderson, K. S., Figueroa, J. D., LaBaer, J. and Qiu, J. (2013), A versatile protein microarray platform enabling antibody profiling against denatured proteins. Prot. Clin. Appl., 7: 378–383. doi: 10.1002/prca.201200062
- Issue published online: 18 JUN 2013
- Article first published online: 17 MAY 2013
- Accepted manuscript online: 2 OCT 2012 05:28AM EST
- Manuscript Accepted: 23 AUG 2012
- Manuscript Revised: 21 AUG 2012
- Manuscript Received: 3 JUL 2012
- Early Detection Research Network. Grant Number: 5U01CA117374–02
- National Cancer Institute, Department of Health and Human Services, USA
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Materials and Methods
1. Detection of plasmid DNA and expressed proteins on HaloTag protein array
2. Blocking of plasma samples with E.coli lysates
3. Protein microarray denaturation
Figure S1. Vector Map of pJFT7-nHalo
Figure S2. Blocking plasma with E. coli lysate can reduce background signal on HeLa IVTT expressed protein array. A. Left, plasma pre-blocked by E. coli lysate; right, no pre-blocking. B. Signal to noise ratio plot of two conditions in A. Background was represented by median signal intensity of all spots on one slide. Feature color represents an artificial color scheme to indicate signal intensity (red > yellow > green > blue).
Figure S3. Different AAb response to TPD52 and MYC between native protein array and denatured protein array.
Table S1. Gene list on HaloTag NAPPA
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