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Improved protein extraction and protein identification from archival formalin-fixed paraffin-embedded human aortas
Version of Record online: 14 APR 2013
© 2013 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim
PROTEOMICS - Clinical Applications
Special Issue: Proteomic Analysis of Formalin Fixed Tissue
Volume 7, Issue 3-4, pages 217–224, April 2013
How to Cite
Fu, Z., Yan, K., Rosenberg, A., Jin, Z., Crain, B., Athas, G., Heide, R. S. V., Howard, T., Everett, A. D., Herrington, D. and Van Eyk, J. E. (2013), Improved protein extraction and protein identification from archival formalin-fixed paraffin-embedded human aortas. Prot. Clin. Appl., 7: 217–224. doi: 10.1002/prca.201200064
- Issue online: 14 APR 2013
- Version of Record online: 14 APR 2013
- Accepted manuscript online: 21 JAN 2013 10:49AM EST
- Manuscript Accepted: 10 DEC 2012
- Manuscript Revised: 7 NOV 2012
- Manuscript Received: 18 JUL 2012
- whole or in part with federal. Grant Numbers: 5RC1HL100021-02, NHLBI-HV-10-05
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|prca1456-sup-0001-TableS1.docx||24K||Supplementary Table 1: Sample characteristics and origins|
|prca1456-sup-0002-TableS2.xlsx||253K||Supplementary Table 2: Identified proteins in unfixed, FF and FFPE aortas using SeQUEST search engine.|
Supplementary Figure 1. Gene ontology analysis of proteins identified with LC-MS/MS and SEQUEST search. Proteins identified using unfixed and FFPE extracted with heat and pressure were categorized by biological process, using GoMiner gene ontology software.
Supplementary Figure 2. MRM detection of MFG-E8 in FFPE Tissue: three peptides (2+), each with three transitions, were detected in unfixed and FFPE aortas extracted with heart and pressure.
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