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Differential proteomic profiling in human spermatozoa that did or did not result in pregnancy via IVF and AID

Authors

  • Yong Zhu,

    1. Department of Urology, Sperm Development and Genetics Laboratory, Renji Hospital, Shanghai Human Sperm Bank, Shanghai Institute of Andrology, Shanghai Jiao Tong University School of Medicine, Shanghai, P. R. China
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    • These authors contributed equally to this work.

  • Yibo Wu,

    1. State Key Laboratory of Reproductive Medicine, Department of Histology and Embryology, Nanjing Medical University, Nanjing, P. R. China
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    • These authors contributed equally to this work.

  • Ke Jin,

    1. Department of Urology, Sperm Development and Genetics Laboratory, Renji Hospital, Shanghai Human Sperm Bank, Shanghai Institute of Andrology, Shanghai Jiao Tong University School of Medicine, Shanghai, P. R. China
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  • Hui Lu,

    1. Department of Urology, Sperm Development and Genetics Laboratory, Renji Hospital, Shanghai Human Sperm Bank, Shanghai Institute of Andrology, Shanghai Jiao Tong University School of Medicine, Shanghai, P. R. China
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  • Feng Liu,

    1. Department of Urology, Sperm Development and Genetics Laboratory, Renji Hospital, Shanghai Human Sperm Bank, Shanghai Institute of Andrology, Shanghai Jiao Tong University School of Medicine, Shanghai, P. R. China
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  • Yueshuai Guo,

    1. State Key Laboratory of Reproductive Medicine, Department of Histology and Embryology, Nanjing Medical University, Nanjing, P. R. China
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  • Feng Yan,

    1. Department of Urology, Sperm Development and Genetics Laboratory, Renji Hospital, Shanghai Human Sperm Bank, Shanghai Institute of Andrology, Shanghai Jiao Tong University School of Medicine, Shanghai, P. R. China
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  • Wenbo Shi,

    1. Department of Urology, Sperm Development and Genetics Laboratory, Renji Hospital, Shanghai Human Sperm Bank, Shanghai Institute of Andrology, Shanghai Jiao Tong University School of Medicine, Shanghai, P. R. China
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  • Yong Liu,

    1. Department of Urology, Sperm Development and Genetics Laboratory, Renji Hospital, Shanghai Human Sperm Bank, Shanghai Institute of Andrology, Shanghai Jiao Tong University School of Medicine, Shanghai, P. R. China
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  • Xiaorong Cao,

    1. Department of Urology, Sperm Development and Genetics Laboratory, Renji Hospital, Shanghai Human Sperm Bank, Shanghai Institute of Andrology, Shanghai Jiao Tong University School of Medicine, Shanghai, P. R. China
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  • Hongliang Hu,

    1. Department of Urology, Sperm Development and Genetics Laboratory, Renji Hospital, Shanghai Human Sperm Bank, Shanghai Institute of Andrology, Shanghai Jiao Tong University School of Medicine, Shanghai, P. R. China
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  • Hui Zhu,

    1. State Key Laboratory of Reproductive Medicine, Department of Histology and Embryology, Nanjing Medical University, Nanjing, P. R. China
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  • Xuejiang Guo,

    1. State Key Laboratory of Reproductive Medicine, Department of Histology and Embryology, Nanjing Medical University, Nanjing, P. R. China
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  • Jiahao Sha,

    1. State Key Laboratory of Reproductive Medicine, Department of Histology and Embryology, Nanjing Medical University, Nanjing, P. R. China
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  • Zheng Li,

    Corresponding author
    1. Department of Urology, Sperm Development and Genetics Laboratory, Renji Hospital, Shanghai Human Sperm Bank, Shanghai Institute of Andrology, Shanghai Jiao Tong University School of Medicine, Shanghai, P. R. China
    • Correspondence: Professor Zheng Li, Department of Urology, Shanghai Institute of Andrology, Renji Hospital, Shanghai Jiaotong University School of Medicine, Shanghai 200001, P. R. China

      E-mail: lizhengboshi@163.com

      Fax: 86-21-6373-2926

      Additional corresponding author: Professor Zuoming Zhou, E-mail: zhouzm@njmu.edu.cn

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  • Zuoming Zhou

    Corresponding author
    1. State Key Laboratory of Reproductive Medicine, Department of Histology and Embryology, Nanjing Medical University, Nanjing, P. R. China
    • Correspondence: Professor Zheng Li, Department of Urology, Shanghai Institute of Andrology, Renji Hospital, Shanghai Jiaotong University School of Medicine, Shanghai 200001, P. R. China

      E-mail: lizhengboshi@163.com

      Fax: 86-21-6373-2926

      Additional corresponding author: Professor Zuoming Zhou, E-mail: zhouzm@njmu.edu.cn

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  • Colour Online: See the article online to view Figs. 1–3 in colour.

Abstract

Purpose

To identify biochemical markers in men with idiopathic infertility and normal sperm counts.

Experimental design

We obtained proteomic profiling proteins in human spermatozoa following successful or unsuccessful pregnancy via assisted reproductive technique (ART) using 6-plex tandem mass tag (TMT) isobaric MS. Our study design consisted of two groups: (i) The semen of six men whose sperm resulted in a clinical pregnancy following ART and (ii) six men whose semen did not result in a clinical pregnancy following ART. The results of differentiated MS were validated by Western blotting.

Results and discussion

A total of 2045 proteins were detected in our cohort. Twenty-one proteins were found to be differentially expressed (>1.2-fold) between Group 1 and Group 2. Using the results of bioinformatics analysis and Western blotting, three proteins (A2LD1, ATP1B3, and FBXO2) were shown to have the same differential pattern (p < 0.05) that was observed in the MS analysis.

Conclusions and clinical relevance

Proteomics may help identity a select cohort of men with normal semen parameters and aid infertility diagnoses.

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