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Proteomic analyses of serous and endometrioid epithelial ovarian cancers – Cases studies – Molecular insights of a possible histological etiology of serous ovarian cancer

Authors

  • Rémi Longuespée,

    1. Université Nord de France, LSMBFA, MALDI Imaging Team, EA 4550, Université de Lille 1, SIRIC ONCOLILLE, Cité Scientifique, Villeneuve D'Ascq, France
    2. Institut de pharmacologie de Sherbrooke et Département de chirurgie/urologie, Faculté de médecine et des sciences de la santé, Université de Sherbrooke, Sherbrooke, Québec, Canada
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    • These are co-authors and have contributed equally to this study.

  • Hugo Gagnon,

    1. Université Nord de France, LSMBFA, MALDI Imaging Team, EA 4550, Université de Lille 1, SIRIC ONCOLILLE, Cité Scientifique, Villeneuve D'Ascq, France
    2. Institut de pharmacologie de Sherbrooke et Département de chirurgie/urologie, Faculté de médecine et des sciences de la santé, Université de Sherbrooke, Sherbrooke, Québec, Canada
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    • These are co-authors and have contributed equally to this study.

  • Charlotte Boyon,

    1. Université Nord de France, LSMBFA, MALDI Imaging Team, EA 4550, Université de Lille 1, SIRIC ONCOLILLE, Cité Scientifique, Villeneuve D'Ascq, France
    2. Hôpital Jeanne de Flandre, service de Chirurgie Gynécologique, CHRU de Lille, France
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  • Kurstin Strupat,

    1. Thermo Fisher Scientific (Bremen) GmbH, Bremen, Germany
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  • Claire Dauly,

    1. Thermo Fisher Scientific (France), Silic, Courtaboeuf, France
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  • Olivier Kerdraon,

    1. Laboratoire d'Anatomie et de Cytologie Pathologiques, CHRU de Lille, Lille, France
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  • Adesuwa Ighodaro,

    1. Université Nord de France, LSMBFA, MALDI Imaging Team, EA 4550, Université de Lille 1, SIRIC ONCOLILLE, Cité Scientifique, Villeneuve D'Ascq, France
    2. OWNIP fellow, SUNY College at Old Westbury, Old Westbury, NY, USA
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  • Annie Desmons,

    1. Université Nord de France, LSMBFA, MALDI Imaging Team, EA 4550, Université de Lille 1, SIRIC ONCOLILLE, Cité Scientifique, Villeneuve D'Ascq, France
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  • Jocelyn Dupuis,

    1. Thermo Fisher Scientific (France), Silic, Courtaboeuf, France
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  • Maxence Wisztorski,

    1. Université Nord de France, LSMBFA, MALDI Imaging Team, EA 4550, Université de Lille 1, SIRIC ONCOLILLE, Cité Scientifique, Villeneuve D'Ascq, France
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  • Denis Vinatier,

    1. Université Nord de France, LSMBFA, MALDI Imaging Team, EA 4550, Université de Lille 1, SIRIC ONCOLILLE, Cité Scientifique, Villeneuve D'Ascq, France
    2. Hôpital Jeanne de Flandre, service de Chirurgie Gynécologique, CHRU de Lille, France
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  • Isabelle Fournier,

    1. Université Nord de France, LSMBFA, MALDI Imaging Team, EA 4550, Université de Lille 1, SIRIC ONCOLILLE, Cité Scientifique, Villeneuve D'Ascq, France
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  • Robert Day,

    1. Institut de pharmacologie de Sherbrooke et Département de chirurgie/urologie, Faculté de médecine et des sciences de la santé, Université de Sherbrooke, Sherbrooke, Québec, Canada
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  • Michel Salzet

    Corresponding author
    • Université Nord de France, LSMBFA, MALDI Imaging Team, EA 4550, Université de Lille 1, SIRIC ONCOLILLE, Cité Scientifique, Villeneuve D'Ascq, France
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  • Colour Online: See the article online to view Figs. 1–7 in colour.

Correspondence: Michel Salzet, Université Nord de France, LSMBFA, MALDI Imaging Team, EA 4550, Université de Lille 1, Cité Scientifique, 59650 Villeneuve D'Ascq, France

E-mail: michel.salzet@univ-lille1.fr

Fax: +3320434054

Abstract

Purpose

Epithelial ovarian carcinogenesis may occur de novo on the surface of ovarian mesothelial epithelial cells or from cells originating in other organs. Foreign Müllerian cell intrusion into the ovarian environment has been hypothesized to explain the latter scenario. In this study, MALDI MS profiling technology was used to provide molecular insights regarding these potentially different mechanisms.

Experimental design

Using MALDI MS profiling, the molecular disease signatures were established in their anatomical context. MALDI MS profiling was used on serous and endometrioid cancer biopsies to investigate cases of epithelial ovarian cancer. We then applied bioinformatic methods and identification strategies on the LC-MS/MS analyses of extracts from digested formalin-fixed, paraffin-embedded tissues. Extracts from selected regions (i.e. serous ovarian adenocarcinoma, fallopian tube serous adenocarcinoma, endometrioid ovarian cancer, benign endometrium, and benign ovarian tissues) were performed, and peptide digests were subjected to LC-MS/MS analysis.

Results

Comparison of the proteins identified from benign endometrium or three ovarian cancer types (i.e. serous ovarian adenocarcinoma, endometrioid ovarian adenocarcinoma, and serous fallopian tube adenocarcinoma) provided new evidence of a possible correlation between the fallopian tubes and serous ovarian adenocarcinoma. Here, we propose a workflow consisting of the comparison of multiple tissues in their anatomical context in an individual patient.

Conclusion and clinical relevance

The present study provides new insights into the molecular similarities between these two tissues and an assessment of highly specific markers for an individualized patient diagnosis and care.

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