Proteomic patterns of colonic mucosal tissues delineate Crohn's colitis and ulcerative colitis
All authors substantially contributed not only to the conception and design but also participated in the acquisition of data, analysis, and interpretation of data and drafting the manuscript.
Correspondence: Dr. Amosy E. M'Koma, Department of Biochemistry and Cancer Biology, Meharry Medical College School of Medicine, 1005 Dr. D. B. Todd Jr. Blvd., Nashville, TN 37208–3599, USA
Although Crohn's colitis (CC) and ulcerative colitis (UC) share several clinical features, they have different causes, mechanisms of tissue damage, and treatment options. Therefore, the accurate diagnosis is of paramount importance in terms of medical care. The distinction between CC/UC is made on the basis of clinical, radiologic, endoscopic, and pathologic interpretations but cannot be differentiated in up to 15% of inflammatory bowel disease patients. Correct management of this “indeterminate colitis” depends on the accuracy of future, and yet not known, destination diagnosis (CC/UC).
We have developed a proteomic methodology that has the potential to discriminate between UC/CC. The histologic layers of 62 confirmed UC/CC tissues were analyzed using MALDI-MS for proteomic profiling.
A Support Vector Machine algorithm consisting of 25 peaks was able to differentiate spectra from CC and UC with 76.9% spectral accuracy when using a leave-20%-out cross-validation. Application of the model to the entire dataset resulted in accurate classification of 19/26 CC patients and 36/36 UC patients when using a 2/3 correct cutoff. A total of 114 peaks were found to have Wilcoxin rank sum p-values of less than 0.05.
Conclusion and clinical relevance
This information may provide new avenues for the development of novel personalized therapeutic targets.