Research Article

Proteomic patterns of colonic mucosal tissues delineate Crohn's colitis and ulcerative colitis

  1. Erin H. Seeley1,
  2. Mary K. Washington2,
  3. Richard M. Caprioli1,3,4,
  4. Amosy E. M'Koma3,5,6,*

Article first published online: 8 MAY 2013

DOI: 10.1002/prca.201200107

Additional Information

How to Cite

Seeley, E. H., Washington, M. K., Caprioli, R. M. and M'Koma, A. E. (2013), Proteomic patterns of colonic mucosal tissues delineate Crohn's colitis and ulcerative colitis. Prot. Clin. Appl.. doi: 10.1002/prca.201200107

Author Information

  1. 1

    Department of Biochemistry, Vanderbilt University School of Medicine, Nashville, TN, USA

  2. 2

    Department of Pathology, Vanderbilt University School of Medicine, Nashville, TN, USA

  3. 3

    Vanderbilt-Ingram Cancer Center, Vanderbilt University School of Medicine, Nashville, TN, USA

  4. 4

    Department of Pharmacology, Medicine and Chemistry, Vanderbilt University School of Medicine, Nashville, TN, USA

  5. 5

    Laboratory of Inflammatory Bowel Disease Research, Department of Biochemistry and Cancer Biology, Meharry Medical College School of Medicine, Nashville, TN, USA

  6. 6

    Department of General Surgery, Vanderbilt University School of Medicine, Nashville, TN, USA

* Correspondence: Dr. Amosy E. M'Koma, Department of Biochemistry and Cancer Biology, Meharry Medical College School of Medicine, 1005 Dr. D. B. Todd Jr. Blvd., Nashville, TN 37208–3599, USA
E-mail: amkoma@mmc.edu
Fax: +1-615-327-6440

  1. All authors substantially contributed not only to the conception and design but also participated in the acquisition of data, analysis, and interpretation of data and drafting the manuscript.

Publication History

  1. Article first published online: 8 MAY 2013
  2. Accepted manuscript online: 4 FEB 2013 08:13AM EST
  3. Manuscript Accepted: 7 JAN 2013
  4. Manuscript Revised: 27 NOV 2012
  5. Manuscript Received: 9 SEP 2012

Funded by

  • MMC/VICC/TSU Partnership PIs: Harold L. Moses and Samuel E. Adunyah. Grant Number: 3U54 CA091405–09S1
  • Vanderbilt CTSA. Grant Number: 5U54RR026140–03
  • NCRR/NIH; Research Foundation, American Society of Colon and Rectal Surgeons. Grant Number: 1 UL1 RR024975
  • DOD. Grant Number: NIH/NCI-CA068485
  • ooperative Human Tissue Network (CHTN). Grant Number: 5U 01CA094664–09
  • Vanderbilt SPORE in GI Cancer. Grant Number: P50CA095103

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Keywords:

  • Colon tissue profiling;
  • Crohn's colitis;
  • Mass spectrometry;
  • Ulcerative colitis

Purpose

Although Crohn's colitis (CC) and ulcerative colitis (UC) share several clinical features, they have different causes, mechanisms of tissue damage, and treatment options. Therefore, the accurate diagnosis is of paramount importance in terms of medical care. The distinction between CC/UC is made on the basis of clinical, radiologic, endoscopic, and pathologic interpretations but cannot be differentiated in up to 15% of inflammatory bowel disease patients. Correct management of this “indeterminate colitis” depends on the accuracy of future, and yet not known, destination diagnosis (CC/UC).

Experimental design

We have developed a proteomic methodology that has the potential to discriminate between UC/CC. The histologic layers of 62 confirmed UC/CC tissues were analyzed using MALDI-MS for proteomic profiling.

Results

A Support Vector Machine algorithm consisting of 25 peaks was able to differentiate spectra from CC and UC with 76.9% spectral accuracy when using a leave-20%-out cross-validation. Application of the model to the entire dataset resulted in accurate classification of 19/26 CC patients and 36/36 UC patients when using a 2/3 correct cutoff. A total of 114 peaks were found to have Wilcoxin rank sum p-values of less than 0.05.

Conclusion and clinical relevance

This information may provide new avenues for the development of novel personalized therapeutic targets.

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