These authors contributed equally to this work.
Trauma-associated human neutrophil alterations revealed by comparative proteomics profiling
Article first published online: 22 MAY 2013
© 2013 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim
PROTEOMICS - Clinical Applications
Special Issue: Focus on Vascular Proteomics
Volume 7, Issue 7-8, pages 571–583, August 2013
How to Cite
Zhou, J.-Y., Krovvidi, R. K., Gao, Y., Gao, H., Petritis, B. O., De, A. K., Miller-Graziano, C. L., Bankey, P. E., Petyuk, V. A., Nicora, C. D., Clauss, T. R., Moore, R. J., Shi, T., Brown, J. N., Kaushal, A., Xiao, W., Davis, R. W., Maier, R. V., Tompkins, R. G., Qian, W.-J., Camp, D. G., Smith, R. D. and the Inflammation and the Host Response to Injury Large Scale Collaborative Research Program (2013), Trauma-associated human neutrophil alterations revealed by comparative proteomics profiling. Prot. Clin. Appl., 7: 571–583. doi: 10.1002/prca.201200109
Colour Online: See the article online to view Figs. 1 and 2 in colour.
- Issue published online: 7 AUG 2013
- Article first published online: 22 MAY 2013
- Accepted manuscript online: 16 APR 2013 03:24AM EST
- Manuscript Accepted: 25 FEB 2013
- Manuscript Revised: 31 JAN 2013
- Manuscript Received: 13 SEP 2012
- NIH. Grant Numbers: U54 GM-62119–02, T32 GM-008256, P41 GM103493, DP2OD006668
- EMSL (Environmental Molecular Science Laboratory
- US Department of Energy (DOE) Office of Biological and Environmental Research on the Pacific Northwest National Laboratory (PNNL) campus in Richland, Washington
- Battelle for the DOE. Grant Number: DE-AC05–76RLO-1830
- Human neutrophil;
Polymorphonuclear neutrophils (PMNs) play an important role in mediating the innate immune response after severe traumatic injury; however, the cellular proteome response to traumatic condition is still largely unknown.
We applied 2D-LC-MS/MS-based shotgun proteomics to perform comparative proteome profiling of human PMNs from severe trauma patients and healthy controls.
A total of 197 out of ∼2500 proteins (being identified with at least two peptides) were observed with significant abundance changes following the injury. The proteomics data were further compared with transcriptomics data for the same genes obtained from an independent patient cohort. The comparison showed that the protein abundance changes for the majority of proteins were consistent with the mRNA abundance changes in terms of directions of changes. Moreover, increased protein secretion was suggested as one of the mechanisms contributing to the observed discrepancy between protein and mRNA abundance changes. Functional analyses of the altered proteins showed that many of these proteins were involved in immune response, protein biosynthesis, protein transport, NRF2-mediated oxidative stress response, the ubiquitin-proteasome system, and apoptosis pathways.
Conclusions and clinical relevance
Our data suggest increased neutrophil activation and inhibited neutrophil apoptosis in response to trauma. The study not only reveals an overall picture of functional neutrophil response to trauma at the proteome level, but also provides a rich proteomics data resource of trauma-associated changes in the neutrophil that will be valuable for further studies of the functions of individual proteins in PMNs.