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Comparative proteomics and correlated signaling network of kidney in ApoE deficient mouse

Authors

  • Xiaoyan Lv,

    1. Department of Dermatology, West China Hospital, West China Medical School, Sichuan University, Chengdu, P. R. China
    2. Core Facility of Genetically Engineered Mice, West China Hospital, West China School of Medicine, Sichuan University, Chengdu, P. R. China
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    • These authors contributed equally to this work.

  • Jianzhong Ai,

    1. Core Facility of Genetically Engineered Mice, West China Hospital, West China School of Medicine, Sichuan University, Chengdu, P. R. China
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    • These authors contributed equally to this work.

  • Mi Li,

    1. Core Facility of Genetically Engineered Mice, West China Hospital, West China School of Medicine, Sichuan University, Chengdu, P. R. China
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  • Honglian Wang,

    1. Core Facility of Genetically Engineered Mice, West China Hospital, West China School of Medicine, Sichuan University, Chengdu, P. R. China
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  • Tielin Chen,

    1. Core Facility of Genetically Engineered Mice, West China Hospital, West China School of Medicine, Sichuan University, Chengdu, P. R. China
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  • Yin Fang,

    1. Core Facility of Genetically Engineered Mice, West China Hospital, West China School of Medicine, Sichuan University, Chengdu, P. R. China
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  • Yunhong Liu,

    1. Core Facility of Genetically Engineered Mice, West China Hospital, West China School of Medicine, Sichuan University, Chengdu, P. R. China
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  • Puhui Zhou,

    1. Core Facility of Genetically Engineered Mice, West China Hospital, West China School of Medicine, Sichuan University, Chengdu, P. R. China
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  • Mianzhi Chen,

    1. Core Facility of Genetically Engineered Mice, West China Hospital, West China School of Medicine, Sichuan University, Chengdu, P. R. China
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  • Ruizhi Tan,

    1. Core Facility of Genetically Engineered Mice, West China Hospital, West China School of Medicine, Sichuan University, Chengdu, P. R. China
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  • Yuhang Liu,

    1. Core Facility of Genetically Engineered Mice, West China Hospital, West China School of Medicine, Sichuan University, Chengdu, P. R. China
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  • Yang Yang,

    1. Core Facility of Genetically Engineered Mice, West China Hospital, West China School of Medicine, Sichuan University, Chengdu, P. R. China
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  • Qin Zhou

    Corresponding author
    1. Core Facility of Genetically Engineered Mice, West China Hospital, West China School of Medicine, Sichuan University, Chengdu, P. R. China
    • Correspondence: Professor Qin Zhou, Core Facility of Genetically Engineered Mice, State Key Laboratory of Biotherapy and Cancer Center, West China Hospital, West China Medical School, Sichuan University, 1# Keyuan 4 Road, Gaopeng Street, Chengdu, 610041, P. R. China

      E-mail: pkdzhou@126.com; zhouqin@scu.edu.cn

      Fax: +86-2-885-125449

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  • Colour Online: See the article online to view Figs. 3 and 5 in colour.

Abstract

Purpose

Apolipoprotein E knockout (apoE−/−) mouse is one of the most popular models for cardiovascular research, especially in the study of atherosclerosis. Naturally, large amount of studies try to uncover the role of apoE in atherosclerosis, and indeed apoE plays an important role in this pathogenesis. Kidney is an organ that contains lots of capillaries and also largely expresses apoE. Moreover, a protective role of apoE in kidney as an autocrine regulator has been demonstrated previously, however, the underlying mechanism is largely unknown.

Experimental design

In this study, comparative proteomics is for the first time used to identify the differential proteins in kidneys of apoE−/− and wild type mice, respectively, and we try to reveal the signaling network of apoE in mice kidney using bioinformatics analysis.

Results

Our findings show that approximately 80 proteins are significantly differentially expressed in kidneys of apoE−/− and wild type mice, and the signaling network correlated to apoE is successfully established by employing bioinformatics assay.

Conclusions and clinical relevance

Taken together, we originally identify the proteins with differential expression and propose an apoE correlated molecular network in mice kidney. These findings further provide evidence of the role of apoE in mice kidney and a brand new perspective in the protection and treatment of kidney disease.

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