Colour Online: See the article online to view Figs. 1, 3, and 4 in colour.
Total serum glycan analysis is superior to lectin-FLISA for the early detection of hepatocellular carcinoma
Article first published online: 13 SEP 2013
© 2013 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim
PROTEOMICS - Clinical Applications
Special Issue: Glycan Biomarker Discovery
Volume 7, Issue 9-10, pages 690–700, October 2013
How to Cite
Comunale, M. A., Wang, M., Anbarasan, N., Betesh, L., Karabudak, A., Moritz, E., Devarajan, K., Marrero, J., Block, T. M. and Mehta, A. (2013), Total serum glycan analysis is superior to lectin-FLISA for the early detection of hepatocellular carcinoma. Prot. Clin. Appl., 7: 690–700. doi: 10.1002/prca.201200125
- Issue published online: 9 OCT 2013
- Article first published online: 13 SEP 2013
- Accepted manuscript online: 16 JUL 2013 03:54AM EST
- Manuscript Accepted: 30 MAR 2013
- Manuscript Revised: 22 FEB 2013
- Manuscript Received: 3 DEC 2012
- National Cancer Institute (NCI). Grant Numbers: R01 CA120206, U01 CA168856
- Hepatitis B Foundation
- Commonwealth of Pennsylvania
- Hepatitis B virus;
- Hepatitis C virus;
- Hepatocellular carcinoma
Hepatocellular carcinoma (HCC) is a primary cancer of the liver that is predominantly the result of infection with a hepatotropic virus such as hepatitis B virus or hepatitis C virus. As liver cancer is often asymptomatic, the development of sensitive noninvasive biomarkers is needed for early detection and improved survival.
We have previously identified alterations in the N-linked glycosylation of serum proteins with the development of HCC and identified many of the proteins that contained the altered glycosylation. In the current study, we compared the ability of the identified proteins to diagnose HCC with the total serum glycan analysis.
Surprisingly, glycan analysis of total serum had the greatest ability to distinguish HCC from cirrhosis with an AUROC of 0.851, a sensitivity of 73% at a specificity of 88%. When total glycan sequencing was combined with alpha-fetoprotein (AFP), the sensitivity increased to 95% at a specificity of 90%.
Conclusion and clinical relevance
Changes in glycosylation as detected in whole serum could be used to diagnose HCC with greater sensitivity and specificity than that observed through the analysis of specific protein glycoforms or protein levels. Such an assay could have value in the management of those at risk for the development of HCC.