The variability in tissue proteomics


  • Visith Thongboonkerd

    Corresponding author
    • Medical Proteomics Unit, Office for Research and Development, Faculty of Medicine Siriraj Hospital, and Center for Research in Complex Systems Science, Mahidol University, Bangkok, Thailand
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Correspondence: Professor Visith Thongboonkerd, Head of Medical Proteomics Unit and Director of Center for Research in Complex Systems Science (CRCSS), Siriraj Hospital, Mahidol University, 6th Floor – SiMR Building, 2 Prannok Road, Bangkoknoi, Bangkok 10700, Thailand


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Variability is one of the most critical issues of concern in clinical proteomics. In this issue of Proteomics Clinical Applications, Yoshida et al. [Proteomics Clin. Appl. 2012, 6, 412–417] describe the effects of blood and number of washes on the human glomerular proteome isolated from the kidney by laser microdissection. The blood-derived proteins occupied almost 50% of all the identified proteins in the unwashed samples, whereas varying the number of washes (from 1–5) with PBS yielded only 43–80% of the proteins identified in each sample that were common in all samples. This urges caution to all proteomists to carefully consider sample preservation and preparation for tissue proteome analysis.