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Aminopeptidase activities as prospective urinary biomarkers for bladder cancer

Authors

  • Jennifer M. Taylor,

    1. Protein Center, Memorial Sloan-Kettering Cancer Center, New York, NY, USA
    2. Department of Surgery, Urology Service, Memorial Sloan-Kettering Cancer Center, New York, NY, USA
    3. Department of Urology, University of Pittsburgh Medical Center, Pittsburgh, PA, USA
    Current affiliation:
    1. Department of Urology, University of Pittsburgh Medical Center, Pittsburgh, PA, USA
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  • Mariana Yaneva,

    1. Protein Center, Memorial Sloan-Kettering Cancer Center, New York, NY, USA
    2. Molecular Biology Program, Memorial Sloan-Kettering Cancer Center, New York, NY, USA
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  • Kevin Velasco,

    1. Protein Center, Memorial Sloan-Kettering Cancer Center, New York, NY, USA
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  • John Philip,

    1. Protein Center, Memorial Sloan-Kettering Cancer Center, New York, NY, USA
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  • Hediye Erdjument-Bromage,

    1. Protein Center, Memorial Sloan-Kettering Cancer Center, New York, NY, USA
    2. Molecular Biology Program, Memorial Sloan-Kettering Cancer Center, New York, NY, USA
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  • Irina Ostrovnaya,

    1. Department of Epidemiology and Biostatistics, Memorial Sloan-Kettering Cancer Center, New York, NY, USA
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  • Hans G. Lilja,

    1. Department of Surgery, Urology Service, Memorial Sloan-Kettering Cancer Center, New York, NY, USA
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  • Bernard H. Bochner,

    1. Department of Surgery, Urology Service, Memorial Sloan-Kettering Cancer Center, New York, NY, USA
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  • Paul Tempst

    Corresponding author
    1. Protein Center, Memorial Sloan-Kettering Cancer Center, New York, NY, USA
    2. Molecular Biology Program, Memorial Sloan-Kettering Cancer Center, New York, NY, USA
    • Correspondence: Dr. Paul Tempst, Memorial Sloan-Kettering Cancer Center, 1275 York Avenue, New York, NY 10065, USA

      E-mail: p-tempst@mskcc.org

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Abstract

Purpose

Proteases have been implicated in cancer progression and invasiveness. We have investigated the activities, as opposed to simple protein levels, of selected aminopeptidases in urine specimens to serve as potential novel biomarkers for urothelial cancer.

Experimental design

The unique urinary proteomes of males and females were profiled to establish the presence of a gender-independent set of aminopeptidases. Samples were also collected from patients with urothelial cancer and matched controls. A SOP for urine processing was developed taking into account hydration variation. Five specific aminopeptidase activity assays, using fluorophoric substrates, were optimized for evaluation of marker potential.

Results

Nineteen exopeptidases and 21 other proteases were identified in urine and the top-five most abundant aminopeptidases, identical in both genders, selected for functional studies. Depending on the enzyme, activities were consistently lower (p ≤ 0.05), higher or unchanged in the cancer samples as compared to controls. Two selected aminopeptidase activities used as a binary classifier resulted in a ROC curve with an AUC = 0.898.

Conclusion and clinical relevance

We have developed functional assays that characterize aminopeptidase activities in urine specimens with adequate technical and intraindividual reproducibility. With further testing, it could yield a reliable biomarker test for bladder cancer detection or prognostication.

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