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Nanoscale elongating control of the self-assembled protein filament with the cysteine-introduced building blocks

  1. Kengo Usui1,2,3,
  2. Tei Maki1,4,
  3. Fuyu Ito1,2,3,
  4. Atsushi Suenaga5,
  5. Satoru Kidoaki4,
  6. Masayoshi Itoh2,3,
  7. Makoto Taiji5,
  8. Takehisa Matsuda6,
  9. Yoshihide Hayashizaki2,3,
  10. Harukazu Suzuki2,3,*

Article first published online: 16 MAR 2009

DOI: 10.1002/pro.106

Issue

Protein Science

Protein Science

Volume 18, Issue 5, pages 960–969, May 2009

Additional Information

How to Cite

Usui, K., Maki, T., Ito, F., Suenaga, A., Kidoaki, S., Itoh, M., Taiji, M., Matsuda, T., Hayashizaki, Y. and Suzuki, H. (2009), Nanoscale elongating control of the self-assembled protein filament with the cysteine-introduced building blocks. Protein Science, 18: 960–969. doi: 10.1002/pro.106

Author Information

  1. 1

    CREST, Japan Science and Technology Agency, Kawaguchi, Saitama 332-0012, Japan

  2. 2

    Laboratory for Genome Exploration Research Group, RIKEN Genomic Sciences Center (GSC), Tsurumi-ku, Yokohama 230-0045, Japan

  3. 3

    Genome Science Laboratory, RIKEN, Hirosawa, Wako 351-0198, Japan

  4. 4

    Division of Biomolecular Chemistry, Kyushu University, Fukuoka 812-8581, Japan

  5. 5

    Computational and Experimental System Biology Group, RIKEN Genomic Sciences Center, Tsurumi, Yokohama, Kanagawa 230-0046, Japan

  6. 6

    Genome Biotechnology Laboratory, Kanazawa Institute of Technology, Ishikawa 924-0838, Japan

*Laboratory for Genome Exploration Research Group, RIKEN Genomic Sciences Center (GSC), 1-7-22 Suehiro-cho, Tsurumi-ku, Yokohama 230-0045, Japan

Publication History

  1. Issue published online: 21 APR 2009
  2. Article first published online: 16 MAR 2009
  3. Accepted manuscript online: 16 MAR 2009 12:00AM EST
  4. Manuscript Accepted: 17 FEB 2009
  5. Manuscript Revised: 16 FEB 2009
  6. Manuscript Received: 3 DEC 2008

Funded by

  • CREST (Core Research for Evolutional Science and Technology) of Japan Science and Technology Corporation
  • RIKEN Genome Exploration Research Project, MEXT of Japanese Government
Corrected by:

Erratum: Nanoscale elongating control of the self-assembled protein filament with the cysteine-introduced building blocks

Vol. 18, Issue 7, 1571, Article first published online: 20 MAY 2009

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Keywords:

  • self-assembly;
  • nanofilament;
  • protein design;
  • molecular modeling;
  • protein nanomaterial

Abstract

Self-assembly of artificially designed proteins is extremely desirable for nanomaterials. Here we show a novel strategy for the creation of self-assembling proteins, named “Nanolego.” Nanolego consists of “structural elements” of a structurally stable symmetrical homo-oligomeric protein and “binding elements,” which are multiple heterointeraction proteins with relatively weak affinity. We have established two key technologies for Nanolego, a stabilization method and a method for terminating the self-assembly process. The stabilization method is mediated by disulfide bonds between Cysteine-residues incorporated into the binding elements, and the termination method uses “capping Nanolegos,” in which some of the binding elements in the Nanolego are absent for the self-assembled ends. With these technologies, we successfully constructed timing-controlled and size-regulated filament-shape complexes via Nanolego self-assembly. The Nanolego concept and these technologies should pave the way for regulated nanoarchitecture using designed proteins.

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