Article
You have full text access to this OnlineOpen article
Racemic crystallography of synthetic protein enantiomers used to determine the X-ray structure of plectasin by direct methods
Article first published online: 7 APR 2009
DOI: 10.1002/pro.127
Copyright © 2009 The Protein Society
Additional Information
How to Cite
Mandal, K., Pentelute, B. L., Tereshko, V., Thammavongsa, V., Schneewind, O., Kossiakoff, A. A. and Kent, S. B. H. (2009), Racemic crystallography of synthetic protein enantiomers used to determine the X-ray structure of plectasin by direct methods. Protein Science, 18: 1146–1154. doi: 10.1002/pro.127
Publication History
- Issue published online: 26 MAY 2009
- Article first published online: 7 APR 2009
- Accepted manuscript online: 7 APR 2009 12:00AM EST
- Manuscript Accepted: 17 MAR 2009
- Manuscript Revised: 8 MAR 2009
- Manuscript Received: 7 JAN 2009
Funded by
- Office of Science (BER), U.S. Department of Energy. Grant Number: DE-FG02-07ER64501
- National Institutes of Health. Grant Number: R01 GM075993
- Office of Science (BER), U.S. Department of Energy. Grant Number: W-31-109-Eng-38
References
- 1, , , , , , , , , , , , , , , , , , , ( 2005) Plectasin is a peptide antibiotic with therapeutic potential from a saprophytic fungus. Nature 437: 975–980.
- 2, , , , , , ( 2008) X-ray structure of snow flea antifreeze protein determined by racemic crystallization of synthetic protein enantiomers. J Am Chem Soc 130: 9695–9701.
- 3, , , , ( 2009) X-ray structure of native scorpion toxin BmBKTx1 by racemic protein crystallography using direct methods. J Am Chem Soc 131: 1362–1363.
- 4, ( 1997) Centrosymmetric crystals of biomolecules: The racemate method. Methods Enzymol 276: 619–627.
- 5, ( 1993) The structure of a centrosymmetric protein crystal. Proteins-Struct Funct Genet 16: 301–305.Direct Link:
- 6, , , ( 1999) Structural differences in D and L-monellin in the crystals of racemic mixture. J Mol Biol 285: 311–321.
- 7, , , , , ( 1994) Conserved and novel structural characteristics of enantiomorphic Leu-enkephalin—X-ray crystal analysis of Leu-enkephalin enantiomer. Int J Pept Protein Res 43: 325–331.Direct Link:
- 8, , , , , ( 1994) Structure determination of racemic trichogin A IV using centrosymmetric crystals. Nat Struct Biol 1: 908–914.
- 9, , , , ( 1999) Centrosymmetric bilayers in the 0.75 angstrom resolution structure of a designed alpha-helical peptide, D,L-Alpha-1. Protein Sci 8: 1410–1422.Direct Link:
- 10, , ( 1992) Total chemical synthesis of a D-enzyme—the enantiomers of HIV-1 Protease demonstrate reciprocal chiral substrate-specificity. Science 256: 1445–1448.
- 11, ( 1992) A racemic protein. J Am Chem Soc 114: 4002–4003.
- 12, , , , ( 2008) Mirror image forms of snow flea antifreeze protein prepared by total chemical synthesis have identical antifreeze activities. J Am Chem Soc 130: 9702–9707.
- 13, , , ( 1994) Synthesis of proteins by native chemical ligation. Science 266: 776–779.
- 14, ( 2000) Synthesis of native proteins by chemical ligation. Annual Rev Biochem 69: 923–960.
- 15, , , , ( 2007) In situ neutralization in Boc-chemistry solid phase peptide synthesis—rapid, high yield assembly of difficult sequences. Int J Pept Res Ther 13: 31–44.
- 16( 1986) The direct methods of X-ray crystallography. Science 233: 178–183.
- 17( 1986) Recovering phase information from intensity data. Science 232: 837–843.
- 18, , , , ( 1993) The application of direct methods and Patterson interpretation to high-resolution native protein data. Acta Crystallogr D Biol Crystallogr 49: 18–23.Direct Link:
- 19, ( 1997) Turbo Frodo, Version Open GL. 1. University Aix-Marseille II: Marseille.
- 20, , ( 1997) Refinement of macromolecular structures by the maximum-likelihood method. Acta Crystallogr D Biol Crystallogr 53: 240–255.Direct Link:
- 21( 2002) The pyMOL molecular graphics system, San Carlos, CA: DeLano Scientific.
- 22( 1994) Ribbons 2.0. J Appl Crystallogr 24: 958–961.Direct Link:
- 23, , , , ( 2008) Research advances in the development of peptide antibiotics. J Pharm Sci 97: 1060–1070.Direct Link:
- 24, ( 1995) Why protein crystals favor some space-groups over others. Nat Struct Biol 2: 1062–1067.
- 25( 1968) Solvent content of protein crystals. J Mol Biol 33: 491–497.
- 26, , , ( 1978) A new synthetic route to tert-butyloxycarbonylaminoacyl-4-(oxymethyl)phenylacetamidomethyl-resin, an improved support for solid-phase peptide synthesis. J Org Chem 43: 2845–2852.
- 27, , , ( 1979) Improved synthesis of 4-(Boc-aminoacyloxymethyl)-phenylacetic acids for use in solid-phase peptide-synthesis. Synthesis 955–957.
- 28, ( 1952) Staphylococcal coagulase; mode of action and antigenicity. J Gen Microbiol 6: 95–107.
- 29, , , , ( 2004) Community-adapted methicillin-resistant Staphylococcus aureus (MRSA): population dynamics of an expanding community reservoir of MRSA. J Infect Dis 190: 1730–1738.
- 30

1469-896X/asset/olbannerleft.gif?v=1&s=d218899ae53b2862ab119790ed504b8d72122fb3)
1469-896X/asset/olbannerright.gif?v=1&s=59470eb9a1d9b7b13b1be75e9445e6c46ee2214f)
