Article
You have full text access to this OnlineOpen article
Structural and functional characterization of a putative polysaccharide deacetylase of the human parasite Encephalitozoon cuniculi
Article first published online: 14 APR 2009
DOI: 10.1002/pro.128
Copyright © 2009 The Protein Society
Additional Information
How to Cite
Urch, J. E., Hurtado-Guerrero, R., Brosson, D., Liu, Z., Eijsink, V. G. H., Texier, C. and van Aalten, D. M. F. (2009), Structural and functional characterization of a putative polysaccharide deacetylase of the human parasite Encephalitozoon cuniculi. Protein Science, 18: 1197–1209. doi: 10.1002/pro.128
Publication History
- Issue published online: 26 MAY 2009
- Article first published online: 14 APR 2009
- Accepted manuscript online: 14 APR 2009 12:00AM EST
- Manuscript Accepted: 30 MAR 2009
- Manuscript Revised: 24 MAR 2009
- Manuscript Received: 13 FEB 2009
Funded by
- Wellcome Trust Senior Research Fellowship
References
- 1, , , , , ( 1999) Microsporidia are related to fungi: evidence from the largest subunit of RNA polymerase II and other proteins. Proc Natl Acad Sci USA 96: 580–585.
- 2, , ( 2000) Evidence from β-tubulin phylogeny that microsporidia evolved from within the fungi. Mol Biol Evol 17: 23–31.
- 3, , ( 2004) Phylogenetic analysis of the complete genome sequence of Encephalitozoon cuniculi supports the fungal origin of microsporidia and reveals a high frequency of fast-evolving genes. J Mol Evol 59: 780–791.
- 4( 2005) Microsporidiosis: an emerging and opportunistic infection in humans and animals. Acta Trop 94: 61–76.
- 5, ( 2002) Microsporidia: biology and evolution of highly reduced intracellular parasites. Annu Rev Microbiol 56: 93–116.
- 6, , , , , , ( 1999) Natural history of intestinal microsporidiosis among patients infected with human immunodeficiency virus. J Clin Microbiol 37: 3421–3422.
- 7, , , , , ( 2001) Intestinal microsporidiosis: a current infection in HIV-seropositive patients in Portugal. Microbes Infect 3: 1015–1019.
- 8, , Clinical syndromes associated with microsporidiosis. In: WittnerM, Ed. ( 1999) The microsporidia and microsporidiosis. Washington, DC: ASM Press, pp 258–292.
- 9, , , , , ( 2000) Phagocytic uptake of Encephalitozoon cuniculi by nonprofessional phagocytes. Infect Immun 68: 6939–6945.
- 10, , , ( 2005) Cell invasion and intracellular fate of Encephalitozoon cuniculi (Microsporidia). Parasitology 130: 285–292.
- 11, , , ( 2005) Apical spore phagocytosis is not a significant route of infection of differentiated enterocytes by Encephalitozoon intestinalis. Infect Immun 73: 7697–7704.
- 12( 2004) Microsporidia: how can they invade other cells? Trends Parasitol 20: 275–279.
- 13, , ( 1999) Effect of low and high temperatures on infectivity of Encephalitozoon cuniculi spores suspended in water. Folia Parasitol (Praha) 46: 171–174.
- 14, ( 1978) Some factors influencing the in vitro infectivity and replication of Encephalitozoon cuniculi. J Protozool 25: 491–496.
- 15, , , , , , ( 1996) Microsporidian spore wall: ultrastructural findings on Encephalitozoon hellem exospore. J Eukaryot Microbiol 43: 181–186.Direct Link:
- 16, Structure of the microsporidia. BullaLAJr and ChengTC Eds. ( 1976) Comparative pathology, Vol. 1. Biology of the microsporidia. London: Plemum Press, pp 1–86.
- 17, , Structure of the microsporidia. In: WittnerM, Ed. ( 1999) The microsporidia and microsporidiosis. Washington, DC: ASM Press, pp 7–84.
- 18, , , , ( 2006) Proteomic analysis of the eukaryotic parasite Encephalitozoon cuniculi (microsporidia): a reference map for proteins expressed in late sporogonial stages. Proteomics 6: 3625–3635.Direct Link:
- 19, , , , ( 2005) The putative chitin deacetylase of Encephalitozoon cuniculi: a surface protein implicated in microsporidian spore-wall formation. FEMS Microbiol Lett 247: 81–90.Direct Link:
- 20, , , ( 2007) Chitosan, the deacetylated form of chitin, is necessary for cell wall integrity in Cryptococcus neoformans. Eukaryot Cell 6: 855–867.
- 21, , , , , , ( 2006) Structure and mechanism of chitin deacetylase from the fungal pathogen Colletotrichum lindemuthianum. Biochemistry 45: 9416–9426.
- 22, , , ( 2005) Structure and metal-dependent mechanism of peptidoglycan deacetylase, a streptococcal virulence factor. Proc Natl Acad Sci USA 102: 15429–15434.
- 23, ( 2004) Structures of Bacillus subtilis PdaA, a family 4 carbohydrate esterase, and a complex with N-acetyl-glucosamine. FEBS Lett 570: 13–19.
- 24, , ( 1993) Bioconversion of chitin to chitosan: purification and characterization of chitin deacetylase from Mucor rouxii. Proc Natl Acad Sci USA 90: 2564–2568.
- 25, , , , , , , , , , , , , ( 2006) Structure and activity of two metal-ion dependent acetyl xylan esterases involved in plant cell wall degradation reveals a close similarity to peptidoglycan deacetylases. J Biol Chem 281: 10968–10975.
- 26, , , , ( 1999) Mode of action of chitin deacetylase from Mucor rouxii on N-acetyl chitooligosaccharides. Eur J Biochem 261: 698–705.Direct Link:
- 27, ( 2000) The pgdA gene encodes for a peptidoglycan N-acetylglucosamine deacetylase in Streptococcus pneumoniae. J Biol Chem 275: 20496– 204501.
- 28, ( 2002) Peptidoglycan N-acetylglucosamine deacetylase, a putative virulence factor in Streptococcus pneumoniae. Infect Immun 70: 7176–7178.
- 29, , , , , , , , , , , , , , , , , , , , ( 2007) A critical role for peptidoglycan N-deacetylation in Listeria evasion from the host innate immune system. Proc Natl Acad Sci USA 104: 997–1002.
- 30, , ( 1996) Two sporulation-specific chitin deacetylase-encoding genes are required for the ascospore wall rigidity of Saccharomyces cerevisiae. J Biol Chem 271: 31420–31425.
- 31, , , ( 1999) Yeast ascospore wall assembly requires two chitin deacetylase isozymes. FEBS Lett 460: 275–279.
- 32, , , , , , , , ( 2007) Major O-glycans in the spores of two microsporidian parasites are represented by unbranched manno-oligosaccharides containing alpha-1,2 linkages. Glycobiology 17: 56–67.
- 33, , , , , , , ( 2002) Ym1 is a neutrophil granule protein that crystallizes in p47phox-deficient mice. J Biol Chem 277: 5468–5475.
- 34, , , ( 2006) The mammalian chitinase-like lectin, YKL-40, binds specifically to type I collagen and modulates the rate of type I collagen fibril formation. J Biol Chem 281: 21082–21095.
- 35, , , , , , , ( 2002) Structure of human chitotriosidase. Implications for specific inhibitor design and function of mammalian chitinase-like lectins. J Biol Chem 277: 25537–25544.
- 36, , , ( 2003) Structure and ligand-induced conformational change of the 39-kDa glycoprotein from human articular chondrocytes. J Biol Chem 278: 30206–30212.
- 37, , , , , , , , , , , , , , , , ( 2001) Genome sequence and gene compaction of the eukaryote parasite Encephalitozoon cuniculi. Nature 414: 450–453.
- 38, , , ( 2002) Developmentally regulated conversion of surface-exposed chitin to chitosan in cell walls of plant pathogenic fungi. New Phytol 156: 103–112.Direct Link:
- 39, , , ( 2004) Expression of chitin deacetylase from Colletotrichum lindemuthianum in Pichia pastoris: purification and characterization. Protein Expr Purif 38: 196–204.
- 40, ( 1997) Processing of X-ray diffraction data collected in oscillation mode. Macromol Crystallogr A 276: 307–326.
- 41, ( 1997) AMoRe: an automated molecular replacement program package. Macromol Crystallogr A 276: 581–594.
- 42, , ( 1999) Automated protein model building combined with iterative structure refinement. Nat Struct Biol 6: 458–463.
- 43, , ( 1997) Refinement of macromolecular structures by the maximum-likelihood method. Acta Crystallogr D Biol Crystallogr 53: 240–255.Direct Link:
- 44, ( 2004) Coot: model-building tools for molecular graphics. Acta Crystallogr D Biol Crystallogr 60: 2126–2132.Direct Link:
- 45, , , ( 2006) Endo/exo mechanism and processivity of family 18 chitinases produced by Serratia Marcescens. FEBS J 273: 491–503.Direct Link:
- 46, ( 2005) Degradation of chitosans with chitinase B from Serratia marcescens. Production of chito-oligosaccharides and insight into enzyme processivity. FEBS J 272: 538–549.Direct Link:
- 47, , , , , , ( 2008) Expression and Characterization of Endochitinase C from Serratia Marcescens BJL200 and Its Purification by One-Step General Chitinase Purification Method. Biosci Biotechnol Biochem 72: 715–723.
- 48, , ( 2005) A colonization factor links Vibrio cholerae environmental survival and human infection. Nature 438( 7069): 863–866.

1469-896X/asset/olbannerleft.gif?v=1&s=d218899ae53b2862ab119790ed504b8d72122fb3)
1469-896X/asset/olbannerright.gif?v=1&s=59470eb9a1d9b7b13b1be75e9445e6c46ee2214f)
