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Cofactor binding and enzymatic activity in an unevolved superfamily of de novo designed 4-helix bundle proteins†

  1. Shona C. Patel1,‡,
  2. Luke H. Bradley2,§,
  3. Sayuri P. Jinadasa2,¶,
  4. Michael H. Hecht2,*

Article first published online: 29 APR 2009

DOI: 10.1002/pro.147

Issue

Protein Science

Protein Science

Volume 18, Issue 7, pages 1388–1400, July 2009

Additional Information

How to Cite

Patel, S. C., Bradley, L. H., Jinadasa, S. P. and Hecht, M. H. (2009), Cofactor binding and enzymatic activity in an unevolved superfamily of de novo designed 4-helix bundle proteins. Protein Science, 18: 1388–1400. doi: 10.1002/pro.147

Author Information

  1. 1

    Department of Chemical Engineering, Princeton University, Princeton, New Jersey 08544

  2. 2

    Department of Chemistry, Princeton University, Princeton, New Jersey 08544

  1. ‡

    Merck & Co., Inc., West Point, PA 19486

  2. §

    Departments of Anatomy & Neurobiology, Molecular & Cellular Biochemistry, University of Kentucky, Lexington, KY 40536

  3. Columbia University Medical Center, New York, NY 10032

*Department of Chemistry, Princeton University, Princeton, NJ, 08544

  1. †

    The authors dedicate this article to the memory of Walter Kauzmann, a long time member of the Princeton Department of Chemistry. His early insights into the importance of the hydrophobic effect in protein folding were decades ahead of their time, and laid the foundation for the protein design strategy described in this article.

Publication History

  1. Issue published online: 23 JUN 2009
  2. Article first published online: 29 APR 2009
  3. Accepted manuscript online: 29 APR 2009 12:00AM EST
  4. Manuscript Accepted: 13 APR 2009
  5. Manuscript Revised: 12 APR 2009
  6. Manuscript Received: 1 MAR 2009

Funded by

  • National Science Foundation Graduate Fellowship
  • Princeton University Council on Science and Technology
  • NSF grant. Grant Number: MCB-0817651

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FilenameFormatSizeDescription
PRO_147_sm_Suppfig-1.tiff327KSupporting Figure 1
PRO_147_sm_Suppfig-2.tiff252KSupporting Figure 2
PRO_147_sm_SuppInfo.doc21KSupplementary Material
PRO_147_sm_SuppTable-1.doc29KTable S1. Summary of HPLC conditions and peak elution times.

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