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Mistic: Cellular localization, solution behavior, polymerization, and fibril formation†

  1. Hay Dvir,
  2. Matthew E. Lundberg,
  3. Samir K. Maji,
  4. Roland Riek,
  5. Senyon Choe*

Article first published online: 29 APR 2009

DOI: 10.1002/pro.148

Issue

Protein Science

Protein Science

Volume 18, Issue 7, pages 1564–1570, July 2009

Additional Information

How to Cite

Dvir, H., Lundberg, M. E., Maji, S. K., Riek, R. and Choe, S. (2009), Mistic: Cellular localization, solution behavior, polymerization, and fibril formation. Protein Science, 18: 1564–1570. doi: 10.1002/pro.148

Author Information

  1. Structural Biology Laboratory, The Salk Institute for Biological Studies, San Diego, California

*Structural Biology Laboratory, The Salk Institute for Biological Studies, 10010 North Torrey Pines Road, La Jolla, CA 92037

  1. †

    Conflict of Interest: A patent on the use of Mistic is filed for membrane protein production in E. coli. Roland Riek is one of the inventors.

Publication History

  1. Issue published online: 23 JUN 2009
  2. Article first published online: 29 APR 2009
  3. Accepted manuscript online: 29 APR 2009 12:00AM EST
  4. Manuscript Accepted: 10 APR 2009
  5. Manuscript Revised: 8 APR 2009
  6. Manuscript Received: 4 FEB 2009

Funded by

  • NIH. Grant Numbers: GM74821,, GM74929

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References

  • 1
    Wallin E, von Heijne G ( 1998) Genome-wide analysis of integral membrane proteins from eubacterial, archaean, and eukaryotic organisms. Protein Sci 7: 1029–1038.
    Direct Link:
  • 2
    Boyd D, Schierle C, Beckwith J ( 1998) How many membrane proteins are there? Protein Sci 7: 201–205.
    Direct Link:
  • 3
    Speers AE, Wu CC ( 2007) Proteomics of integral membrane proteins—theory and application. Chem Rev 107: 3687–3714.
  • 4
    Roosild TP, Greenwald J, Vega M, Castronovo S, Riek R, Choe S ( 2005) NMR structure of Mistic, a membrane-integrating protein for membrane protein expression. Science 307: 1317–1321.
  • 5
    Roosild TP, Vega M, Castronovo S, Choe S ( 2006) Characterization of the family of Mistic homologues. BMC Struct Biol 6: 10.
  • 6
    Kefala G, Kwiatkowski W, Esquivies L, Maslennikov I, Choe S ( 2007) Application of Mistic to improving the expression and membrane integration of histidine kinase receptors from Escherichia coli. J Struct Funct Genomics 8: 167–172.
  • 7
    Liu X, Wu L, Deng G, Li N, Chu X, Guo F, Li D ( 2008) Characterization of mitochondrial trifunctional protein and its inactivation study for medicine development. Biochim Biophys Acta 1784: 1742–1749.
  • 8
    Lee KE, Kim HM, Lee JO, Jeon H, Han SS ( 2008) Regulation of CD40 reconstitution into a liposome using different ratios of solubilized LDAO to lipids. Colloids Surf B: Biointerfaces 62: 51–57.
  • 9
    Matthews BW ( 1968) Solvent content of protein crystals. J Mol Biol 33: 491–497.
  • 10
    Vagin A, Teplyakov A ( 1997) MOLREP: an automated program for molecular replacement. J Appl Cryst 30: 1022–1025.
    Direct Link:
  • 11
    CCP4 ( 1994) Collaborative Computational Project, Number 4. Acta Cryst D Biol Cryst 50: 760–763.
  • 12
    Naiki H, Higuchi K, Hosokawa M, Takeda T ( 1989) Fluorometric determination of amyloid fibrils in vitro using the fluorescent dye, thioflavin T1. Anal Biochem 177: 244–249.
  • 13
    Bennett MJ, Schlunegger MP, Eisenberg D ( 1995) 3D domain swapping: a mechanism for oligomer assembly. Protein Sci 4: 2455–2468.
    Direct Link:
  • 14
    Guo Z, Eisenberg D ( 2006) Runaway domain swapping in amyloid-like fibrils of T7 endonuclease I. Proc Natl Acad Sci USA 103: 8042–8047.
  • 15
    Bennett MJ, Choe S, Eisenberg D ( 1994) Refined structure of dimeric diphtheria toxin at 2.0 Å resolution. Protein Sci 3: 1444–1463.
    Direct Link:
  • 16
    McLean LR, Balasubramaniam A ( 1992) Promotion of beta-structure by interaction of diabetes associated polypeptide (amylin) with phosphatidylcholine. Biochim Biophys Acta 1122: 317–320.
  • 17
    Mirzabekov TA, Lin MC, Kagan BL ( 1996) Pore formation by the cytotoxic islet amyloid peptide amylin. J Biol Chem 271: 1988–1992.
  • 18
    Anderluh G, Gutierrez-Aguirre I, Rabzelj S, Ceru S, Kopitar-Jerala N, Macek P, Turk V, Zerovnik E ( 2005) Interaction of human stefin B in the prefibrillar oligomeric form with membranes. Correlation with cellular toxicity. FEBS J 272: 3042–3051.
    Direct Link:
  • 19
    Kagan BL, Hirakura Y, Azimov R, Azimova R, Lin MC ( 2002) The channel hypothesis of Alzheimer's disease: current status. Peptides 23: 1311–1315.
  • 20
    Leslie AGW ( 1992) Recent changes to the MOSFLM package for processing film and image plate data. Joint CCP4 + ESF-EAMCB Newsletter on Protein Crystallography, No. 26.
  • 21
    Evans PR ( 1997) Scala. Joint CCP4 + ESF-EAMCB Newsletter on Protein Crystallography 33: 22–24.
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