Justus Hammon and Dinesh V. Palanivelu contributed equally to this work.
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A green fluorescent protein screen for identification of well-expressed membrane proteins from a cohort of extremophilic organisms
Article first published online: 2 DEC 2008
DOI: 10.1002/pro.18
Copyright © 2008 The Protein Society
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How to Cite
Hammon, J., Palanivelu, D. V., Chen, J., Patel, C. and Minor, D. L. (2009), A green fluorescent protein screen for identification of well-expressed membrane proteins from a cohort of extremophilic organisms. Protein Science, 18: 121–133. doi: 10.1002/pro.18
Publication History
- Issue published online: 16 DEC 2008
- Article first published online: 2 DEC 2008
- Accepted manuscript online: 2 DEC 2008 12:00AM EST
- Manuscript Accepted: 15 OCT 2008
- Manuscript Revised: 10 OCT 2008
- Manuscript Received: 3 SEP 2008
Funded by
- NIH-NIGMS (Membrane Protein Expression Center and Center for the Structures of Membrane Proteins)
- American Heart Association
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Additional Supporting Information may be found in the online version of this article.
| Filename | Format | Size | Description |
|---|---|---|---|
| PRO_18_sm_SuppFiguresandTables.pdf | 272K | Supporting Information Figure 1. Diagram of the LIC vectors used in this study. In each case the restriction site used to prepare the LIC site in the vector is shown in light blue. Supporting Information Figure 2. Examples of construct and temperature optimization for two candidates, Sp1 and Sp2. GFP indicates the GFP-fusion construct for each protein. H3C, HM3C, and CH indicate N-terminal His8, N-terminal His6-maltose binding protein, and C-terminal His8 versions, respectively. Western blots are probed with an anti-polyhistidine antibody. Expression temperatures are indicated. Supporting Information Table 1. Candidate membrane proteins. Supporting Information Table 2. Size exclusion apparent molecular weights for monodisperse samples. |
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