Detection of disordered regions in globular proteins using 13C-detected NMR

Authors

  • Felicia L. V. Gray,

    1. Department of Pathology, University of Michigan, Ann Arbor, Michigan 48109
    2. Program in Chemical Biology, University of Michigan, Ann Arbor, Michigan 48109
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  • Marcelo J. Murai,

    1. Department of Pathology, University of Michigan, Ann Arbor, Michigan 48109
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  • Jolanta Grembecka,

    1. Department of Pathology, University of Michigan, Ann Arbor, Michigan 48109
    2. Program in Chemical Biology, University of Michigan, Ann Arbor, Michigan 48109
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  • Tomasz Cierpicki

    Corresponding author
    1. Department of Pathology, University of Michigan, Ann Arbor, Michigan 48109
    2. Program in Chemical Biology, University of Michigan, Ann Arbor, Michigan 48109
    • Department of Pathology, University of Michigan, 1150 W. Medical Center Drive, MSRB1 4516, Ann Arbor, MI 48109
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Abstract

Characterization of disordered regions in globular proteins constitutes a significant challenge. Here, we report an approach based on 13C-detected nuclear magnetic resonance experiments for the identification and assignment of disordered regions in large proteins. Using this method, we demonstrate that disordered fragments can be accurately identified in two homologs of menin, a globular protein with a molecular weight over 50 kDa. Our work provides an efficient way to characterize disordered fragments in globular proteins for structural biology applications.

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