Additional Supporting Information may be found in the online version of this article.

PRO_2223_sm_SuppFig1.tif3204KSupporting Information Figure 1. PCR screen to ensure genomic integration of expression cassette for human PEMT (A), mouse PEMT (B) and yeast PEMT (OPI3) (C). Lane numbers refer to colony number picked from plates in Fig 2. Positions 1 and 2 are negative controls (untransformed GS115).
PRO_2223_sm_SuppFig2.tif285KSupporting Information Figure 2. Size exclusion chromatography of purified mPEMT injected onto a Superdex 200 16/160 column equilibrated in 20 mM Tris pH 8.0, 0.15M NaCl, 5% glycerol, 0.15% FC-12. The protein eluted as a single monodisperse peak with minimal aggregation. Elution for standard proteins: 1. Thyroglobulin 50.7ml (MW, 670kDa; Stokes radius 85 Å); 2. γ-globulin 66.8ml (MW 158kDa; Stokes radius 52.9 Å); 3. Ovalbumin 66.8ml (MW 44kDa; Stokes radius 30.5 Å); 4. myoglobin 93.4ml (MW 17kDa, Stokes radius 20.7 Å) ; 5. vitamin B12 100.7ml (MW 1.3kDa, Stokes radius 1.6 Å).

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