• 1
    Gräslund S, Nordlund P, Weigelt J, Hallberg BM, Bray J, Gileadi O, Knapp S, Oppermann U, Arrowsmith C, Hui R, Ming J, dhe-Paganon S, Park HW, Savchenko A, Yee A, Edwards A, Vincentelli R, Cambillau C, Kim R, Kim SH, Rao Z, Shi Y, Terwilliger TC, Kim CY, Hung LW, Waldo GS, Peleg Y, Albeck S, Unger T, Dym O, Prilusky J, Sussman JL, Stevens RC, Lesley SA, Wilson IA, Joachimiak A, Collart F, Dementieva I, Donnelly MI, Eschenfeldt WH, Kim Y, Stols L, Wu R, Zhou M, Burley SK, Emtage JS, Sauder JM, Thompson D, Bain K, Luz J, Gheyi T, Zhang F, Atwell S, Almo SC, Bonanno JB, Fiser A, Swaminathan S, Studier FW, Chance MR, Sali A, Acton TB, Xiao R, Zhao L, Ma LC, Hunt JF, Tong L, Cunningham K, Inouye M, Anderson S, Janjua H, Shastry R, Ho CK, Wang D, Wang H, Jiang M, Montelione GT, Stuart DI, Owens RJ, Daenke S, Schütz A, Heinemann U, Yokoyama S, Büssow K, Gunsalus KC (2008) Protein production and purification. Nat Methods 5:135146.
  • 2
    Terpe K (2003) Overview of tag protein fusions: from molecular and biochemical fundamentals to commercial systems. Appl Microbiol Biotechnol 60:523533.
  • 3
    Lichty JJ, Malecki JL, Agnew HD, Michelson-Horowitz DJ, Tan S (2005) Comparison of affinity tags for protein purification. Protein Expr Purif 41:98105.
  • 4
    Eiberle MK, Jungbauer A (2010) Technical refolding of proteins: do we have freedom to operate? Biotechnol J 5:547559.
  • 5
    Reinhard C, Hebert SS, De Strooper B (2005) The amyloid-beta precursor protein: integrating structure with biological function. EMBO J 24:39964006.
  • 6
    Gralle M, Ferreira ST (2007) Structure and functions of the human amyloid precursor protein: the whole is more than the sum of its parts. Prog Neurobiol 82:1132.
  • 7
    Tabata S, Nampo M, Mihara E, Tamura-Kawakami K, Fujii I, Takagi J (2010) A rapid screening method for cell lines producing singly-tagged recombinant proteins using the "TARGET tag" system. J Proteomics 73:17771785.
  • 8
    Takeuchi J, Tamura T (2004) Recombinant ATPases of the yeast 26S proteasome activate protein degradation by the 20S proteasome. FEBS Lett 565:3942.
  • 9
    Chow MK, Amin AA, Fulton KF, Fernando T, Kamau L, Batty C, Louca M, Ho S, Whisstock JC, Bottomley SP, Buckle AM (2006) The REFOLD database: a tool for the optimization of protein expression and refolding. Nucleic Acids Res 34:D207D212.
  • 10
    Austin BP, Tozser J, Bagossi P, Tropea JE, Waugh DS (2011) The substrate specificity of Metarhizium anisopliae and Bos taurus carboxypeptidases A: insights into their use as tools for the removal of affinity tags. Protein Expr Purif 77:5361.
  • 11
    Austin BP, Waugh DS (2012) Isolation of Metarhizium anisopliae carboxypeptidase A with native disulfide bonds from the cytosol of Escherichia coli BL21(DE3). Protein Expr Purif 82:116124.
  • 12
    Joshi L, St Leger RJ (1999) Cloning, expression, and substrate specificity of MeCPA, a zinc carboxypeptidase that is secreted into infected tissues by the fungal entomopathogen Metarhizium anisopliae. J Biol Chem 274:98039811.
  • 13
    Carvalho CM, Aires-Barros MR, Cabral JM (1999) Cutinase: from molecular level to bioprocess development. Biotechnol Bioeng 66:1734.
  • 14
    Goncalves AM, Serro AP, Aires-Barros MR, Cabral JM (2000) Effects of ionic surfactants used in reversed micelles on cutinase activity and stability. Biochim Biophys Acta 1480:92106.
  • 15
    Hayashi I, Takatori S, Urano Y, Miyake Y, Takagi J, Sakata-Yanagimoto M, Iwanari H, Osawa S, Morohashi Y, Li T, Wong PC, Chiba S, Kodama T, Hamakubo T, Tomita T, Iwatsubo T (2012) Neutralization of the gamma-secretase activity by monoclonal antibody against extracellular domain of nicastrin. Oncogene 31:787798.
  • 16
    Nagae M, Re S, Mihara E, Nogi T, Sugita Y, Takagi J (2012) Crystal structure of alpha5beta1 integrin ectodomain: atomic details of the fibronectin receptor. J Cell Biol 197:131140.
  • 17
    Sun W, Xie J, Lin H, Mi S, Li Z, Hua F, Hu Z (2012) A combined strategy improves the solubility of aggregation-prone single-chain variable fragment antibodies. Protein Expr Purif 83:2129.
  • 18
    Nogi T, Sangawa T, Tabata S, Nagae M, Tamura-Kawakami K, Beppu A, Hattori M, Yasui N, Takagi J (2008) Novel affinity tag system using structurally defined antibody–tag interaction: application to single-step protein purification. Protein Sci 17:21202126.
  • 19
    Hedhammar M, Graslund T, Uhlen M, Hober S (2004) Negatively charged purification tags for selective anion-exchange recovery. Protein Eng Des Sel 17:779786.
  • 20
    Waugh DS (2005) Making the most of affinity tags. Trends Biotechnol 23:316320.
  • 21
    Ohlson M, Sorensson J, Lindstrom K, Blom AM, Fries E, Haraldsson B (2001) Effects of filtration rate on the glomerular barrier and clearance of four differently shaped molecules. Am J Physiol Renal Physiol 281:F103F113.
  • 22
    Takagi J, Erickson HP, Springer TA (2001) C-terminal opening mimics "inside–out" activation of integrin a5b1. Nature Struct Biol 8:412416.