Membrane phospholipid bilayer as a determinant of monoacylglycerol lipase kinetic profile and conformational repertoire
Article first published online: 29 APR 2013
© 2013 The Protein Society
Volume 22, Issue 6, pages 774–787, June 2013
How to Cite
Nasr, M. L., Shi, X., Bowman, A. L., Johnson, M., Zvonok, N., Janero, D. R., Vemuri, V. K., Wales, T. E., Engen, J. R. and Makriyannis, A. (2013), Membrane phospholipid bilayer as a determinant of monoacylglycerol lipase kinetic profile and conformational repertoire. Protein Science, 22: 774–787. doi: 10.1002/pro.2257
- Issue published online: 25 MAY 2013
- Article first published online: 29 APR 2013
- Accepted manuscript online: 30 MAR 2013 01:23AM EST
- Manuscript Accepted: 19 MAR 2013
- Manuscript Revised: 7 MAR 2013
- Manuscript Received: 17 JAN 2013
- National Institutes of Health; Grant numbers: DA003801 and DA009158 (to AM); Grant sponsor: National Institute on Drug Abuse; Grant numbers: GM08607 and GM101135 (to JRE); Grant sponsors: National Institute of General Medical Sciences and by a research collaboration with Waters Corporation (to JRE)
Additional Supporting Information may be found in the online version of this article.
|pro2257-sup-0001-suppinfo.docx||885K||Figure S1. Analysis of hMGL (green line), POPC/POPG phospholipid bilayer nanodiscs (red line), and hMGL-nanodisc mixture (black line) by (a) size-exclusion FPLC and (b) SDS-PAGE. The calibration scale for the size-exclusion column is shown and was derived from proteins of known Stokes hydrodynamic diameter: thyroglobulin (bovine thyroid) (17 nm), ferritin (horse spleen) (12.2 nm), catalase (bovine liver) (10.4 nm), albumin (bovine serum) (7.1 nm).|
Please note: Wiley Blackwell is not responsible for the content or functionality of any supporting information supplied by the authors. Any queries (other than missing content) should be directed to the corresponding author for the article.