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Using affinity chromatography to engineer and characterize pH-dependent protein switches
Article first published online: 2 DEC 2008
DOI: 10.1002/pro.23
Copyright © 2008 The Protein Society
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How to Cite
Sagermann, M., Chapleau, R. R., DeLorimier, E. and Lei, M. (2009), Using affinity chromatography to engineer and characterize pH-dependent protein switches. Protein Science, 18: 217–228. doi: 10.1002/pro.23
Publication History
- Issue published online: 16 DEC 2008
- Article first published online: 2 DEC 2008
- Accepted manuscript online: 2 DEC 2008 12:00AM EST
- Manuscript Accepted: 23 OCT 2008
- Manuscript Revised: 21 OCT 2008
- Manuscript Received: 21 AUG 2008
References
- 1, ( 2005) Allosteric regulation of chaperonins. Curr Opin Struct Biol 15: 646–651.
- 2, ( 2001) The guanidine nucleotide binding switch in three dimensionss. Science 294: 1299–1304.
- 3, , , , ( 2003) Allosteric determinants in guanine nucleotide-binding proteins. Proc Natl Acad Sci USA 100: 14445–14450.
- 4( 2004) Allostery and coupled sequence variation in nuclear hormone receptors. Cell 116: 354–356.
- 5, ( 2006) The changing landscape of protein allostery. Curr Opin Struct Biol 16: 102–108.
- 6, , , ( 1994) Structure of influenza haemagglutinin at the pH of membrane fusion. Nature 371: 37–43.
- 7, ( 1993) A spring-loaded mechanism for the conformational change of influenza hemagglutinin. Cell 73: 823–832.
- 8, ( 2004) The 70-kDa heat shock protein chaperone nucleotide-binding domain in solution unveiled as a molecular machine that can reorient its functional subdomains. Proc Natl Acad Sci USA 101: 10272–10277.
- 9, , ( 1996) Structure of a mutant adenylate kinase ligated with an ATP-analogue showing domain closure over ATP. J Mol Biol 256: 223–227.
- 10( 1960) There is plenty of room at the bottom. Eng Sci (Caltech) 23: 22–36.
- 11, ( 2005) Peptides as novel smart materials. Curr Opin Struct Biol 15: 453–463.
- 12( 2003) Fabrication of novel biomaterials through molecular self-assembly. Nat Biotechnol 21: 1171–1178.
- 13, , , , , ( 2004) Sequence and structural duality: designing peptides to adopt two stable conformations. J Am Chem Soc 126: 17016–17024.
- 14, ( 2005) Computational design of a single amino acid sequence that can switch between two distinct protein folds. J Am Chem Soc 128: 1154–1161.
- 15, ( 2004) Structural changes linked to proton translocation by subunit c of the ATPsynthase. Nature 402: 263–268.
- 16, , , ( 2001) Structure, function and evolution of glutathione transferases: implications for classification of non-mammalian members of an ancient enzyme superfamily. Biochem J 360: 1–16.
- 17, , , , , , ( 2005) Crystallographic and thermodynamic analysis of the binding of S-octylglutathione to the Tyr 7 to Phe mutant of glutathione S-transferase from Schistosoma japonicum. Biochemistry 44: 1174–1183.
- 18, , , , , , ( 1995) Site-directed mutagenesis of human glutathione transferase P1-1. J Biol Chem 270: 1243–1248.
- 19, ( 2006) An intersubunit lock-and-key ‘clasp’ motif in the dimer interface of Delta class glutathione transferase. Biochem J 394: 135–144.
- 20, , , , ( 1991) Structural and thermodynamic consequences of burying a charged residue within the hydrophobic core of T4 lysozyme. Biochemistry 30: 11521–11529.
- 21., , ( 1995) Substitution of charged residues into the hydrophobic core of Escherichia coli thioredoxin results in a change in heat capacity of the native protein. Biochemistry 34: 2148–2152.
- 22, , , ( 1991) In a staphylococcal nuclease mutant the side-chain of a lysine replacing valine 66 is fully buried in the hydrophobic core. J Mol Biol 221: 7–14.
- 23, , , , , ( 1993) Energetic cost and structural consequences of burying a hydroxyl group within the core of a protein determined from Ala→Ser and Val→Thr substitutions in T4 lysozyme. Biochemistry 32: 11363–11373.
- 24, , , ( 1993) The role of backbone flexibility in the accommodation of variants that repack the core of T4 lysozyme. Science 262: 1715–1718.
- 25, , , , , , , , ( 2000) Buried charged surface in proteins. Structure 8: 1203–1214.
- 26, , ( 2005) Very fast empirical prediction and interpretation of protein pKa values. Proteins 61: 704–721.Direct Link:
- 27, , ( 1991) Are the histidine residues of glutathione-S-transferase important in catalysis? J Biol Chem 266: 19475–19479.
- 28, ( 2007) Affinity-purification mass spectrometry (AP-MS) of serine/threonine phosphatases. Methods 42: 298–305.
- 29, , , , , ( 1999) A generic protein purification method for protein complex characterization and proteome exploration. Nat Biotechnol 17: 1030–1032.
- 30, , , , ( 2004) Discovery of an allosteric site in the caspases. Proc Natl Acad Sci USA 101: 12461–12466.
- 31, , ( 2006) A common allosteric site and mechanism in caspases. Proc Natl Acad Sci USA 103: 7595–7600.
- 32, , , , , ( 2008) Electrostatic effects in a network of polar and ionizable groups in staphylococcal nuclease. J Mol Biol 379: 1045–1062.
- 33, , ( 2002) De novo design of biocatalysts. Curr Opin Chem Biol 6: 125–129.
- 34, ( 2007) Progress in computational protein design. Curr Opin Biotechnol 18: 305–311.
- 35, , , , , , , , , , , , , ( 2008) De novo computational design of retro-aldol enzymes. Science 319: 1387–1391.
- 36, ( 2008) Allosteric effects in the marginally stable von Hippel-Lindau tumor suppressor protein and allostery-based rescue mutant design. Proc Natl Acad Sci USA 105: 901–906.
- 37, ( 2004) Searching for new allosteric sites in enzymes. Curr Opin Struct Biol 14: 706–715.
- 38( 1988) Evaluation of single-crystal X-ray diffraction data from a position-sensitive detector. J Appl Crystallogr 21: 916–924.Direct Link:
- 39( 1993) Automatic processing of rotation diffraction data from crystals of initially unknown symmetry and cell constants. J Appl Crystallogr 26: 795–800.Direct Link:
- 40, , , , , , ( 1994) Three-dimensional structure of Schistosoma japonicum glutathione S-transferase fused with a six-amino acid conserved neutralizing epitope of gp41 from HIV. Protein Sci 3: 2233–2244.Direct Link:
- 41Collaborative Computational Project Number 4 ( 1994) The CCP4 suite: programs for protein crystallography. Acta Crystallogr D Biol Cryst 50: 760–763.Direct Link:
- 42
- 43, , , , , , , , , ( 1998) Crystallography & NMR system: a new software suite for macromolecular structure determination. Acta Crystallogr D 54: 905–921.Direct Link:

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