Article

You have full text access to this OnlineOpen article

Structural differences between apolipoprotein E3 and E4 as measured by 19F NMR

  1. Kanchan Garai,
  2. Sourajit M. Mustafi,
  3. Berevan Baban,
  4. Carl Frieden*

Article first published online: 10 NOV 2009

DOI: 10.1002/pro.283

Issue

Protein Science

Protein Science

Volume 19, Issue 1, pages 66–74, January 2010

Additional Information

How to Cite

Garai, K., Mustafi, S. M., Baban, B. and Frieden, C. (2010), Structural differences between apolipoprotein E3 and E4 as measured by 19F NMR. Protein Science, 19: 66–74. doi: 10.1002/pro.283

Author Information

  1. Department of Biochemistry and Molecular Biophysics, Washington University School of Medicine, St. Louis, Missouri 63110

*Department of Biochemistry and Molecular Biophysics, Washington University School of Medicine, 660 S. Euclid Av, St. Louis, MO 63110

Publication History

  1. Issue published online: 6 JAN 2010
  2. Article first published online: 10 NOV 2009
  3. Accepted manuscript online: 10 NOV 2009 12:00AM EST
  4. Manuscript Accepted: 23 OCT 2009
  5. Manuscript Received: 27 AUG 2009

Funded by

  • NIH. Grant Number: 5R01 DK13332

SEARCH

SEARCH BY CITATION

ARTICLE TOOLS

View Full Article with Supporting Information (HTML) Get PDF (287K)

References

  • 1
    Aggerbeck LP, Wetterau JR, Weisgraber KH, Wu CS, Lindgren FT ( 1988) Human apolipoprotein E3 in aqueous solution. II. Properties of the amino- and carboxyl-terminal domains. J Biol Chem 263: 6249–6258.
  • 2
    Chou CY, Lin YL, Huang YC, Sheu SY, Lin TH, Tsay HJ, Chang GG, Shiao MS ( 2005) Structural variation in human apolipoprotein E3 and E4: secondary structure, tertiary structure, and size distribution. Biophys J 88: 455–466.
  • 3
    Hatters DM, Peters-Libeu CA, Weisgraber KH ( 2006) Apolipoprotein E structure: insights into function. Trends Biochem Sci 31: 445–454.
  • 4
    Perugini MA, Schuck P, Howlett GJ ( 2000) Self-association of human apolipoprotein E3 and E4 in the presence and absence of phospholipid. J Biol Chem 275: 36758–36765.
  • 5
    Yokoyama S, Kawai Y, Tajima S, Yamamoto A ( 1985) Behavior of human apolipoprotein E in aqueous solutions and at interfaces. J Biol Chem 260: 16375–16382.
  • 6
    Wilson C, Wardell MR, Weisgraber KH, Mahley RW, Agard DA ( 1991) Three-dimensional structure of the LDL receptor-binding domain of human apolipoprotein E. Science 252: 1817–1822.
  • 7
    Sivashanmugam A, Wang J ( 2009) A unified scheme for initiation and conformational adaptation of human apolipoprotein E N-terminal domain upon lipoprotein-binding and for receptor-binding activity. J Biol Chem 284: 14657–14666.
  • 8
    Weisgraber KH ( 1994) Apolipoprotein E: structure-function relationships. Adv Protein Chem 45: 249–302.
  • 9
    Segrest JP, Jones MK, De Loof H, Brouillette CG, Venkatachalapathi YV, Anantharamaiah GM ( 1992) The amphipathic helix in the exchangeable apolipoproteins: a review of secondary structure and function. J Lipid Res 33: 141–166.
  • 10
    Zhang Y, Vasudevan S, Sojitrawala R, Zhao W, Cui C, Xu C, Fan D, Newhouse Y, Balestra R, Jerome WG, Weisgraber K, Li Q, Wang J ( 2007) A monomeric, biologically active, full-length human apolipoprotein E. Biochemistry 46: 10722–10732.
  • 11
    Zhang Y, Chen J, Wang J ( 2008) A complete backbone spectral assignment of lipid-free human apolipoprotein E (apoE). Biomol NMR Assign 2: 207–210.
  • 12
    Bann JG, Pinkner J, Hultgren SJ, Frieden C ( 2002) Real-time and equilibrium (19)F-NMR studies reveal the role of domain-domain interactions in the folding of the chaperone PapD. Proc Natl Acad Sci USA 99: 709–714.
  • 13
    Frieden C ( 2003) The kinetics of side chain stabilization during protein folding. Biochemistry 42: 12439– 12446.
  • 14
    Frieden C, Hoeltzli SD, Bann JG ( 2004) The preparation of 19F-labeled proteins for NMR studies. Methods Enzymol 380: 400–415.
  • 15
    Hoeltzli SD, Frieden C ( 1994) 19F NMR spectroscopy of [6-19F]tryptophan-labeled E. coli dihydrofolate reductase: equilibrium folding and ligand binding studies. Biochemistry 33: 5502–5509.
  • 16
    Hoeltzli SD, Frieden C ( 1996) Real-time refolding studies of 6-19F-tryptophan labeled Escherichia coli dihydrofolate reductase using stopped-flow NMR spectroscopy. Biochemistry 35: 16843–16851.
  • 17
    Li H, Frieden C ( 2005) NMR studies of 4-19F-phenylalanine-labeled intestinal fatty acid binding protein: evidence for conformational heterogeneity in the native state. Biochemistry 44: 2369–2377.
  • 18
    Santoro MM, Bolen DW ( 1988) Unfolding free energy changes determined bu the linear extrapolation method. 1. Unfolding of phenylmethanesulfonyl alpha-chymotrypsin using different denaturants. Biochemistry 27: 8063–8068.
  • 19
    Ropson IJ, Frieden C ( 1992) Dynamic NMR spectral analysis and protein folding: identification of a highly populated folding intermediate of rat intestinal fatty acid-binding protein by 19F NMR. Proc Natl Acad Sci USA 89: 7222–7226.
  • 20
    Corder EH, Woodbury MA ( 1993) Genetic heterogeneity in Alzheimer's disease: a grade of membership analysis. Genet Epidemiol 10: 495–499.
    Direct Link:
  • 21
    Corder EH, Saunders AM, Strittmatter WJ, Schmechel DE, Gaskell PC, Small GW, Roses AD, Haines JL, Pericak-Vance MA ( 1993) Gene dose of apolipoprotein E type 4 allele and the risk of Alzheimer's disease in late onset families. Science 261: 921–923.
  • 22
    Drzezga A, Grimmer T, Henriksen G, Muhlau M, Perneczky R, Miederer I, Praus C, Sorg C, Wohlschlager A, Riemenschneider M, Wester HJ, Foerstl H, Schwaiger M, Kurz A ( 2009) Effect of APOE genotype on amyloid plaque load and gray matter volume in Alzheimer disease. Neurology 72: 1487–1494.
  • 23
    Farrer LA, Cupples LA, Haines JL, Hyman B, Kukull WA, Mayeux R, Myers RH, Pericak-Vance MA, Risch N, Van Duijn CM ( 1997) Effects of age, sex, and ethnicity on the association between apolipoprotein E genotype and Alzheimer disease. A meta-analysis. APOE and Alzheimer disease meta analysis consortium. JAMA 278: 1349–1356.
  • 24
    Saunders AM, Strittmatter WJ, Schmechel D, George-Hyslop PH, Pericak-Vance MA, Joo SH, Rosi BL, Gusella JF, Crapper-Maclachlan DR, Alberts MJ ( 1993) Association of apolipoprotein E allele epsilon 4 with late-onset familial and sporadic Alzheimer's disease. Neurology 43: 1467–1472.
  • 25
    Strittmatter WJ, Roses AD ( 1995) Apolipoprotein E and Alzheimer disease. Proc Natl Acad Sci USA 92: 4725–4727.
  • 26
    Sakamoto T, Tanaka M, Vedhachalam C, Nickel M, Nguyen D, Dhanasekaran P, Phillips MC, Lund-Katz S, Saito H ( 2008) Contributions of the carboxyl-terminal helical segment to the self-association and lipoprotein preferences of human apolipoprotein E3 and E4 isoforms. Biochemistry 47: 2968–2977.
  • 27
    Hatters DM, Peters-Libeu CA, Weisgraber KH ( 2005) Engineering conformational destabilization into mouse apolipoprotein E. A model for a unique property of human apolipoprotein E4. J Biol Chem 280: 26477–26482.
  • 28
    Hatters DM, Budamagunta MS, Voss JC, Weisgraber KH ( 2005) Modulation of apolipoprotein E structure by domain interaction: differences in lipid-bound and lipid-free forms. J Biol Chem 280: 34288–34295.
View Full Article with Supporting Information (HTML) Get PDF (287K)