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Coordination sphere of the third metal site is essential to the activity and metal selectivity of alkaline phosphatases†

  1. Dimitris Koutsioulis1,2,
  2. Andrzej Lyskowski3,
  3. Seija Mäki3,
  4. Ellen Guthrie1,
  5. Georges Feller4,
  6. Vassilis Bouriotis2,
  7. Pirkko Heikinheimo3,*

Article first published online: 13 NOV 2009

DOI: 10.1002/pro.284

Issue

Protein Science

Protein Science

Volume 19, Issue 1, pages 75–84, January 2010

Additional Information

How to Cite

Koutsioulis, D., Lyskowski, A., Mäki, S., Guthrie, E., Feller, G., Bouriotis, V. and Heikinheimo, P. (2010), Coordination sphere of the third metal site is essential to the activity and metal selectivity of alkaline phosphatases. Protein Science, 19: 75–84. doi: 10.1002/pro.284

Author Information

  1. 1

    New England Biolabs Inc., 240 County Road, Ipswich, Massachusetts 01938-2723

  2. 2

    Department of Biology, University of Crete, Heraklion, Crete, Greece

  3. 3

    Structural Biology and Biophysics, Institute of Biotechnology, University of Helsinki, Helsinki, FIN-00014 Finland

  4. 4

    Laboratory of Biochemistry, Centre for Protein Engineering, University of Liège, Institute of Chemistry B6a, Liège-Sart Tilman, Belgium

*Structural Biology and Biophysics, Institute of Biotechnology, PO Box 65, University of Helsinki, Helsinki, FIN-00014 Finland

  1. †

    Some of this work was supported by New England Biolabs, Inc., a company that may profit from the sale of enzymes described herein. The authors are (EPG) or were (DK) employees of New England Biolabs, Inc.

Publication History

  1. Issue published online: 6 JAN 2010
  2. Article first published online: 13 NOV 2009
  3. Accepted manuscript online: 13 NOV 2009 12:00AM EST
  4. Manuscript Accepted: 23 OCT 2009
  5. Manuscript Revised: 7 SEP 2009
  6. Manuscript Received: 6 JUL 2009

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