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Mutational analysis of active-site residues in the Mycobacterium leprae RecA intein, a LAGLIDADG homing endonuclease: Asp122 and Asp193 are crucial to the double-stranded DNA cleavage activity whereas Asp218 is not

  1. Pawan Singh,
  2. Pankaj Tripathi,
  3. K. Muniyappa*

Article first published online: 20 NOV 2009

DOI: 10.1002/pro.292

Issue

Protein Science

Protein Science

Volume 19, Issue 1, pages 111–123, January 2010

Additional Information

How to Cite

Singh, P., Tripathi, P. and Muniyappa, K. (2010), Mutational analysis of active-site residues in the Mycobacterium leprae RecA intein, a LAGLIDADG homing endonuclease: Asp122 and Asp193 are crucial to the double-stranded DNA cleavage activity whereas Asp218 is not. Protein Science, 19: 111–123. doi: 10.1002/pro.292

Author Information

  1. Department of Biochemistry, Indian Institute of Science, Bangalore 560012, Karnataka, India

*Department of Biochemistry, Indian Institute of Science, Bangalore 560012, Karnataka, India

Publication History

  1. Issue published online: 6 JAN 2010
  2. Article first published online: 20 NOV 2009
  3. Accepted manuscript online: 20 NOV 2009 12:00AM EST
  4. Manuscript Accepted: 20 OCT 2009
  5. Manuscript Revised: 19 OCT 2009
  6. Manuscript Received: 4 SEP 2009

Funded by

  • Department of Biotechnology, New Delhi (Centre of Excellence)
  • J. C. Bose National Fellowship

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