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Radiation inactivation of galactose oxidase, a monomeric enzyme with a stable free radical
Article first published online: 8 DEC 2009
DOI: 10.1002/pro.302
Copyright © 2009 The Protein Society
Additional Information
How to Cite
Kempner, E. S., Whittaker, J. W. and Miller, J. H. (2010), Radiation inactivation of galactose oxidase, a monomeric enzyme with a stable free radical. Protein Science, 19: 236–241. doi: 10.1002/pro.302
Publication History
- Issue published online: 21 JAN 2010
- Article first published online: 8 DEC 2009
- Accepted manuscript online: 8 DEC 2009 12:00AM EST
- Manuscript Accepted: 23 NOV 2009
- Manuscript Revised: 19 NOV 2009
- Manuscript Received: 7 SEP 2009
Funded by
- NIH. Grant Number: GM046749
- Intramural Research Program of NIAMS
References
- 1, ( 1998) Protein radicals in catalysis. Chem Rev 98: 705–762.
- 2( 2003) Free radical catalysis by galactose oxidase. Chem Rev 103: 2347–2364.
- 3, , , ( 2009) Control of radical chemistry in the AdoMet radical enzymes. Curr Opin Chem Biol 13: 74–83.
- 4, , ( 2006) Free radical mechanisms in enzymology. Chem Rev 106: 3302–3316.
- 5( 1999) Advances in radiation target analysis. Anal Biochem 276: 113–123.
- 6, , , , , , ( 2000) Radiation inactivation of ribonucleotide reductase, an enzyme with a stable free radical. Biophys J 79: 2155–2161.
- 7
- 8, The free radical-coupled copper active site of galactose oxidase. In: SigelH, SigelA, Eds. ( 1994) Metal ions in biological systems, Vol. 30. New York: Dekker, pp 315–360.
- 9, , , ( 1994) Crystal structure of a free radical enzyme, galactose oxidase. J Mol Biol 236: 794–814.
- 10, , , , ( 1991) Inactivation mechanism of tetrameric β-galactosidase by γ-rays involves both fragmentation and temperature-dependent denaturation of protomers. Biochemistry 30: 8151–8157.
- 11, , , , ( 1998) Radiation effects on the native structure of proteins: fragmentation without dissociation. Arch Biochem Biophys 352: 281–287.
- 12, ( 1986) Radiation inactivation of assimilatory NADH:nitrate reductase from Chlorella. J Biol Chem 261: 806–810.
- 13, , , ( 1987) Radiation inactivation analysis of assimilatory NADH:nitrate reductase. J Biol Chem 262: 8934–8939.
- 14, , ( 1986) Radiation inactivation probe of membrane-bound enzymes: γ- glutamyltranspeptidase, aminopeptidase N and sucrase. Anal Biochem 158: 278–282.
- 15, , , , ( 1987) Radiation inactivation analysis of enzymes. J Biol Chem 262: 9433–9436.
- 16, ( 1989) Radiation-damaged tyrosinase molecules are inactive. Biophys J 55: 159–162.
- 17, , , ( 1974) The molecular properties of the copper enzyme galactose oxidase. Arch Biochem Biophys 165: 456–467.
- 18, , , , , , , , ( 1985) Target size of 5′-nucleotidase in smooth muscle. J Biochem 98: 573–575.
- 19, , , ( 1985) Target size of Ca-pumps in pig coronary artery smooth muscle. Life Sci 37: 2193–2198.
- 20, , ( 1986) Muscarinic receptor size on smooth muscle cells and membranes. Am J Physiol 251: G195–G200.
- 21, , ( 1986) Determination of the molecular weight of neuronectin, a conditioned medium-derived, substrate-binding neurite-extension factor: comparison with laminin using radiation-inactivation analysis. J Neurosci 6: 1563– 1569.
- 22, , , , , ( 1987) Target size analysis of neurotensin receptors. Peptides 8: 195–197.
- 23, , , , ( 2002) Pulse radiolysis studies of galactose oxidase. Inorg Chem 41: 2158–2163.
- 24( 1958) On the enzymatic activity of subtilisin-modified ribonuclease. Proc Natl Acad Sci USA 33: 162–166.
- 25, ( 1976) The proteolytic nature of commercial samples of galactose oxidase. Biochim Biophys Acta 438: 339–346.
- 26, ( 1990) A tyrosine-derived free radical in apogalactose oxidase. J Biol Chem 265: 9610–9613.
- 27, ( 1990) Direct effects of radiation on the avidin-biotin complex. J Biol Chem 265: 15776–15781.
- 28, Worthington Manual, 1993 edition. p 177.
- 29, , , , , , , , ( 1985) Measurement of protein using bicinchoninic acid. Anal Biochem 150: 76–85.
- 30, ( 1982) Galactose oxidase from Dactylium dendroides. Meth Enzymol 89: 163–171.
- 31, , ( 1985) Molecular weight determinations from radiation inactivation. Meth Enzymol 117: 65–94.

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