Radiation inactivation of galactose oxidase, a monomeric enzyme with a stable free radical

doi:10.1002/pro.302

Article

You have full text access to this OnlineOpen article

Radiation inactivation of galactose oxidase, a monomeric enzyme with a stable free radical

Ellis S. Kempner^1,*,
James W. Whittaker²,
Jay H. Miller¹

Article first published online: 8 DEC 2009

DOI: 10.1002/pro.302

Issue

Protein Science

Volume 19, Issue 2, pages 236–241, February 2010

Additional Information

How to Cite

Kempner, E. S., Whittaker, J. W. and Miller, J. H. (2010), Radiation inactivation of galactose oxidase, a monomeric enzyme with a stable free radical. Protein Science, 19: 236–241. doi: 10.1002/pro.302

Author Information

¹
Laboratory of Physical Biology, National Institute of Arthritis and Musculoskeletal and Skin Diseases, National Institutes of Health, Bethesda, Maryland 20892
²
Department of Science and Engineering, School of Medicine, Oregon Health and Sciences University, Beaverton, Oregon 97006

Email: Ellis S. Kempner (eskempner@yahoo.com)

^*Laboratory of Physical Biology, National Institute of Arthritis and Musculoskeletal and Skin Diseases, National Institutes of Health, Bethesda, MD 20892

Publication History

Issue published online: 21 JAN 2010
Article first published online: 8 DEC 2009
Accepted manuscript online: 8 DEC 2009 12:00AM EST
Manuscript Accepted: 23 NOV 2009
Manuscript Revised: 19 NOV 2009
Manuscript Received: 7 SEP 2009

Funded by

NIH. Grant Number: GM046749
Intramural Research Program of NIAMS

ARTICLE TOOLS

View Full Article (HTML) Get PDF (188K)

References

1
Stubbe J, van der Donk WA ( 1998) Protein radicals in catalysis. Chem Rev 98: 705–762.
2
Whittaker JW ( 2003) Free radical catalysis by galactose oxidase. Chem Rev 103: 2347–2364.
3
Duschene KS, Veneziano SE, Silver SC, Broderick JB ( 2009) Control of radical chemistry in the AdoMet radical enzymes. Curr Opin Chem Biol 13: 74–83.
4
Frey PA, Hegeman AD, Reed GH ( 2006) Free radical mechanisms in enzymology. Chem Rev 106: 3302–3316.
5
Kempner ES ( 1999) Advances in radiation target analysis. Anal Biochem 276: 113–123.
6
Bolger GT, Liuzzi M, Krogsrud R, Scouten E, McCollum RS, Welchner E, Kempner ES ( 2000) Radiation inactivation of ribonucleotide reductase, an enzyme with a stable free radical. Biophys J 79: 2155–2161.
7
Knowles PF, Ito N ( 1993) Galactose oxidase. Perspect Bioinorg Chem 2: 207–244.
- CAS
8
Whittaker JW, The free radical-coupled copper active site of galactose oxidase. In: SigelH, SigelA, Eds. ( 1994) Metal ions in biological systems, Vol. 30. New York: Dekker, pp 315–360.
- Web of Science® Times Cited: 127
9
Ito N, Phillips SEV, Yadav KDS, Knowles PF ( 1994) Crystal structure of a free radical enzyme, galactose oxidase. J Mol Biol 236: 794–814.
10
Potier M, Thauvette L, Michaud L, Giroux S, Beauregard G ( 1991) Inactivation mechanism of tetrameric β-galactosidase by γ-rays involves both fragmentation and temperature-dependent denaturation of protomers. Biochemistry 30: 8151–8157.
11
Miller JH, Fedoronko DA, Hass BD, Myint M, Kempner ES ( 1998) Radiation effects on the native structure of proteins: fragmentation without dissociation. Arch Biochem Biophys 352: 281–287.
12
Solomonson LP, McCreery MJ ( 1986) Radiation inactivation of assimilatory NADH:nitrate reductase from Chlorella. J Biol Chem 261: 806–810.
13
Solomonson LP, McCreery MJ, Kay CJ, Barber MJ ( 1987) Radiation inactivation analysis of assimilatory NADH:nitrate reductase. J Biol Chem 262: 8934–8939.
14
Stevens BR, Kempner ES, Wright EM ( 1986) Radiation inactivation probe of membrane-bound enzymes: γ- glutamyltranspeptidase, aminopeptidase N and sucrase. Anal Biochem 158: 278–282.
15
Eichler DC, Solomonson LP, Barber MJ, McCreery MJ, Ness GC ( 1987) Radiation inactivation analysis of enzymes. J Biol Chem 262: 9433–9436.
16
Kempner ES, Miller JH ( 1989) Radiation-damaged tyrosinase molecules are inactive. Biophys J 55: 159–162.
17
Kosman DL, Ettinger MJ, Weiner RE, Massaro EJ ( 1974) The molecular properties of the copper enzyme galactose oxidase. Arch Biochem Biophys 165: 456–467.
18
Grover AK, Agrawal DK, Ahmad S, Daniel EE, Kwan C-Y, Oakes PJ, Sipos SN, Berenski C, Jung CY ( 1985) Target size of 5′-nucleotidase in smooth muscle. J Biochem 98: 573–575.
19
Grover AK, Samson SE, Berenski C, Jung CY ( 1985) Target size of Ca-pumps in pig coronary artery smooth muscle. Life Sci 37: 2193–2198.
20
Collins SM, Jung CY, Grover AK ( 1986) Muscarinic receptor size on smooth muscle cells and membranes. Am J Physiol 251: G195–G200.
21
Coughlin MD, Grover AK, Jung CY ( 1986) Determination of the molecular weight of neuronectin, a conditioned medium-derived, substrate-binding neurite-extension factor: comparison with laminin using radiation-inactivation analysis. J Neurosci 6: 1563– 1569.
22
Ahmad S, Kwan CY, Vincent JP, Grover, AK, Jung CY, Daniel EE ( 1987) Target size analysis of neurotensin receptors. Peptides 8: 195–197.
23
Borman CD, Wright C, Twitchett MB, Salmon GA, Sykes AG ( 2002) Pulse radiolysis studies of galactose oxidase. Inorg Chem 41: 2158–2163.
24
Richards FM ( 1958) On the enzymatic activity of subtilisin-modified ribonuclease. Proc Natl Acad Sci USA 33: 162–166.
25
Hatton MWC, Regoeczi E ( 1976) The proteolytic nature of commercial samples of galactose oxidase. Biochim Biophys Acta 438: 339–346.
26
Whittaker MM, Whittaker JW ( 1990) A tyrosine-derived free radical in apogalactose oxidase. J Biol Chem 265: 9610–9613.
27
Kempner ES, Miller JH ( 1990) Direct effects of radiation on the avidin-biotin complex. J Biol Chem 265: 15776–15781.
28
Worthington V, Worthington Manual, 1993 edition. p 177.
29
Smith PK, Krohn RI, Hermanson GT, Mallia AK, Gartner FH, Provenzano MD, Fujimoto EK, Olson BJ, Klenk DC ( 1985) Measurement of protein using bicinchoninic acid. Anal Biochem 150: 76–85.
30
Tressel PS, Kosman DL ( 1982) Galactose oxidase from Dactylium dendroides. Meth Enzymol 89: 163–171.
31
Harmon JT, Nielsen TB, Kempner ES ( 1985) Molecular weight determinations from radiation inactivation. Meth Enzymol 117: 65–94.

View Full Article (HTML) Get PDF (188K)

JOURNAL TOOLS

JOURNAL MENU

FIND ISSUES

FIND ARTICLES

GET ACCESS

FOR CONTRIBUTORS

ABOUT THIS JOURNAL

SPECIAL FEATURES

Radiation inactivation of galactose oxidase, a monomeric enzyme with a stable free radical

Protein Science

How to Cite

Author Information

Publication History

Funded by

ARTICLE TOOLS

References

More content like this

JOURNAL TOOLS

JOURNAL MENU

FIND ISSUES

FIND ARTICLES

GET ACCESS

FOR CONTRIBUTORS

ABOUT THIS JOURNAL

SPECIAL FEATURES

Radiation inactivation of galactose oxidase, a monomeric enzyme with a stable free radical

Protein Science

How to Cite

Author Information

Publication History

Funded by

SEARCH

SEARCH BY CITATION

ARTICLE TOOLS

References

More content like this