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Osmolytes modulate conformational exchange in solvent-exposed regions of membrane proteins
Article first published online: 10 DEC 2009
DOI: 10.1002/pro.305
Copyright © 2009 The Protein Society
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How to Cite
Flores Jiménez, R. H., Do Cao, M.-A., Kim, M. and Cafiso, D. S. (2010), Osmolytes modulate conformational exchange in solvent-exposed regions of membrane proteins. Protein Science, 19: 269–278. doi: 10.1002/pro.305
Publication History
- Issue published online: 21 JAN 2010
- Article first published online: 10 DEC 2009
- Accepted manuscript online: 10 DEC 2009 12:00AM EST
- Manuscript Accepted: 4 DEC 2009
- Manuscript Revised: 18 NOV 2009
- Manuscript Received: 23 SEP 2009
Funded by
- National Institutes of Health, NIGMS. Grant Number: GM 035215
References
- 1, , ( 1991) The energy landscapes and motions of proteins. Science 254: 1598–1603.
- 2, ( 2007) Dynamic personalities of proteins. Nature 450: 964–972.
- 3, ( 2006) The changing landscape of protein allostery. Curr Opin Struct Biol 16: 102–108.
- 4, , ( 2007) Intrinsic dynamics of enzymes in the unbound state and relation to allosteric regulation. Curr Opin Struct Biol 17: 633–640.
- 5, , , , , ( 2007) A hierarchy of timescales in protein dynamics is linked to enzyme catalysis. Nature 450: 913–916.
- 6
- 7, , , , ( 2005) Flexible nets. The roles of intrinsic disorder in protein interaction networks. FEBS J 272: 5129–5148.Direct Link:
- 8, , , ( 1998) Recent advances in site-directed spin labeling of proteins. Curr Opin Struct Biol 8: 649–656.
- 9, , ( 2000) Identifying conformational changes with site-directed spin labeling. Nat Struct Biol 7: 735–739.
- 10, ( 2006) Recent advances and applications of site-directed spin labeling. Curr Opin Struct Biol 16: 644–653.
- 11, , , ( 1996) Motion of spin-labeled side-chains in T4 lysozyme. I. Correlation with protein structure and dynamics. Biochemistry 35: 7692–7704.
- 12, ( 2002) A new spin on protein dynamics. Trends Biochem Sci 27: 288–295.
- 13, ( 2004) Mapping backbone dynamics in solution with site-directed spin labeling: GCN4-58 bZip free and bound to DNA. Biochemistry 43: 7273–7287.
- 14, , , ( 2003) Substrate-induced transmembrane signaling in the cobalamin transporter BtuB. Nat Struct Biol 10: 394–401.
- 15, , , , , ( 2006) In meso structure of the cobalamin transporter, BtuB, at 1.95 A resolution. J Mol Biol 364: 716–734.
- 16, , , ( 2006) Substrate-dependent unfolding of the energy coupling motif of a membrane transport protein determined by double electron-electron resonance. Biochemistry 45: 10847–10854.
- 17, ( 2003) Touch and go: tying TonB to transport. Mol Microbiol 49: 869–882.Direct Link:
- 18, , ( 2003) Spectroscopic evidence that osmolytes used in crystallization buffers inhibit a conformation change in a membrane protein. Biochemistry 42: 13106–13112.
- 19, , , ( 2006) Solutes modify a conformational transition in a membrane transport protein. Biophys J 90: 2922–2929.
- 20, , , , ( 2007) Structural determinants of nitroxide motion in spin-labeled proteins: tertiary contact and solvent-inaccessible sites in helix G of T4 lysozyme. Protein Sci 16: 1069–1086.Direct Link:
- 21, , , , ( 2001) Molecular motion of spin labeled side chains in a-helices: analysis by variation of side chain structure. Biochemistry 40: 3228–3846.
- 22, , , , , ( 2003) Substrate-induced conformational changes of the perplasmic N-terminus of an outer-membrane transporter by site-directed spin labeling. Biochemistry 42: 1391–1400.
- 23, ( 1985) Mechanism of poly(ethylene glycol) interaction with proteins. Biochemistry 24: 6756–6762.
- 24, , , , ( 2009) Osmolyte perturbation reveals conformational equilibria in spin-labeled proteins. Protein Sci 18: 1637–1652.Direct Link:
- 25, , , ( 2008) Solutes alter the conformation of the ligand binding loops in outer membrane transporters. Biochemistry 47: 670–679.
- 26, , ( 2003) Membrane mimetic environments alter the conformation of the outer membrane protein BtuB. J Am Chem Soc 125: 13932–13933.
- 27( 2002) Protein hydration, thermodynamic binding, and preferential hydration. Biochemistry 41: 13473–13482.
- 28, , ( 2007) Substrate-dependent transmembrane signaling in TonB-dependent transporters is not conserved. Proc Natl Acad Sci USA 104: 11975–11980.
- 29, ( 2003) Preferential interactions of urea with lysozyme and their linkage to protein denaturation. Biophys Chem 105: 421–448.
- 30( 2004) Effects of naturally occurring osmolytes on protein stability and solubility: issues important in protein crystallization. Methods 34: 312–322.
- 31( 1998) Control of protein stability and reactions by weakly interacting cosolvents: the simplicity of the complicated. Adv Protein Chem 51: 355–432.
- 32, , ( 1990) Solute inaccessible aqueous volume changes during opening of the potassium channel of the squid giant axon. Biophys J 57: 1049–1064.
- 33, , ( 1992) Protein solvation in allosteric regulation: a water effect on hemoglobin. Science 256: 1335–1336.
- 34, , ( 2004) Conformational changes associated with protein-protein interactions. Curr Opin Struct Biol 14: 104–109.
- 35, , ( 2004) Complementarity of structure ensembles in protein-protein binding. Structure 12: 2125–2136.
- 36, ( 2008) Conformer selection and induced fit in flexible backbone protein-protein docking using computational and NMR ensembles. J Mol Biol 381: 1068–1087.
- 37, , ( 1993) Probing alamethicin channels with water-soluble polymers. Size-modulated osmotic action. Biophys J 65: 2097–2105.
- 38, , ( 1995) Macromolecules and water: probing with osmotic stress. Methods Enzymol 259: 43–94.
- 39
- 40, , , , ( 1998) Coupling site-directed mutagenesis with high-level expression: large scale production of mutant porins from E. coli. FEMS Microbiol Lett 163: 65–72.Direct Link:
- 41, ( 1985) Nucleotide sequence of the gene for the vitamin B12 receptor protein in the outer membrane of Escherichia coli. J Bacteriol 161: 904–908.
- 42, , , , ( 2001) Transport-defective mutations alter the conformation of the energy-coupling motif of an outer membrane transporter. Biochemistry 40: 13946–13971.
- 43, , , , ( 2002) Structure and dynamics of the β-barrel of the membrane transporter BtuB by site-directed spin labeling. Biochemistry 41: 11543–11551.
- 44, ( 1990) Interdependence of calcium and cobalamin binding by wild-type and mutant BtuB protein in the outer membrane of Escherichia coli. J Bacterial 172: 4919–4926.
- 45, , ( 2005) Protein folding, stability, and solvation structure in osmolyte solutions. Biophys J 89: 2988–2997.

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