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Elucidating residue roles in engineered variants of AraC regulatory protein
Article first published online: 15 DEC 2009
DOI: 10.1002/pro.310
Copyright © 2009 The Protein Society
Additional Information
How to Cite
Tang, S.-Y. and Cirino, P. C. (2010), Elucidating residue roles in engineered variants of AraC regulatory protein. Protein Science, 19: 291–298. doi: 10.1002/pro.310
Publication History
- Issue published online: 21 JAN 2010
- Article first published online: 15 DEC 2009
- Accepted manuscript online: 15 DEC 2009 12:00AM EST
- Manuscript Accepted: 1 DEC 2009
- Manuscript Revised: 30 NOV 2009
- Manuscript Received: 10 SEP 2009
Funded by
- The National Science Foundation. Grant Number: 0644678
References
- 1, , ( 1990) Characterization of the Escherichia coli araFGH and araJ promoters. J Mol Biol 215: 497–510.
- 2, ( 1978) Nucleotide sequence of L-arabinose regulatory region of Escherichia coli K12. J Biol Chem 253: 6931–6933.
- 3, , ( 1980) The araC gene of Escherichia coli: transcriptional and translational start-points and complete nucleotide sequence. Gene 12: 179–190.
- 4( 2003) AraC protein: a love-hate relationship. Bioessays 25: 274–282.Direct Link:
- 5, , ( 1998) Arm-domain interactions in AraC. J Mol Biol 278: 539–548.
- 6, ( 1998) Apo-AraC actively seeks to loop. J Mol Biol 278: 529–538.
- 7, ( 1999) Hemiplegic mutations in AraC protein. J Mol Biol 294: 417–425.
- 8, ( 2001) Mapping arm-DNA-binding domain interactions in AraC. J Mol Biol 307: 1001–1009.
- 9, ( 1993) Functional domains of the AraC protein. Proc Natl Acad Sci USA 90: 5638–5642.
- 10, , ( 1994) Reaching out. Locating and lengthening the interdomain linker in AraC protein. J Mol Biol 242: 330–338.
- 11, , , ( 1997) Structural basis for ligand-regulated oligomerization of AraC. Science 276: 421–425.
- 12, ( 2001) Strengthened arm-dimerization domain interactions in AraC. J Biol Chem 276: 2562–2564.
- 13, , ( 2003) Mutational analysis of residue roles in AraC function. J Mol Biol 328: 85–93.
- 14, , ( 2008) AraC regulatory protein mutants with altered effector specificity. J Am Chem Soc 130: 5267–5271.
- 15, , ( 1989) Studies on the domain structure of the Salmonella typhimurium AraC protein. Eur J Biochem 185: 319–325.Direct Link:
- 16, , , ( 2007) Structure and properties of a truly apo form of AraC dimerization domain. Proteins 66: 646–654.Direct Link:
- 17, , ( 2009) Starch and alpha-glucan acting enzymes, modulating their properties by directed evolution. J Biotechnol 140: 184–193.
- 18, , , , Library construction for protein engineering. In: ParkSJ, CochranJR, Eds. ( 2010) Protein engineering and design. CRC Press, pp 83–108.
- 19, , , ( 2004) Directed evolution of human estrogen receptor variants with significantly enhanced androgen specificity and affinity. J Biol Chem 279: 33855–33864.
- 20, , , , ( 2008) In vitro DNA recombination by L-Shuffling during ribosome display affinity maturation of an anti-Fas antibody increases the population of improved variants. Protein Eng Des Sel 21: 343–351.
- 21, ( 2001) Biophysical evidence of arm-domain interactions in AraC. Anal Biochem 295: 107–112.
- 22, , , ( 2009) Functional modes of the regulatory arm of AraC. Proteins 74: 81–91.Direct Link: