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Cyclic voltammetry: A new strategy for the evaluation of oxidative damage to bovine insulin

  1. Wansong Zong,
  2. Rutao Liu*,
  3. Feng Sun,
  4. Meijie Wang,
  5. Pengjun Zhang,
  6. Yihong Liu,
  7. Yanmin Tian

Article first published online: 21 DEC 2009

DOI: 10.1002/pro.313

Issue

Protein Science

Protein Science

Volume 19, Issue 2, pages 263–268, February 2010

Additional Information

How to Cite

Zong, W., Liu, R., Sun, F., Wang, M., Zhang, P., Liu, Y. and Tian, Y. (2010), Cyclic voltammetry: A new strategy for the evaluation of oxidative damage to bovine insulin. Protein Science, 19: 263–268. doi: 10.1002/pro.313

Author Information

  1. School of Environmental Science and Engineering, Shandong University, Jinan 250100, People's Republic of China

*School of Environmental Science and Engineering, Shandong University, Jinan 250100, People's Republic of China

Publication History

  1. Issue published online: 21 JAN 2010
  2. Article first published online: 21 DEC 2009
  3. Accepted manuscript online: 21 DEC 2009 12:00AM EST
  4. Manuscript Accepted: 27 NOV 2009
  5. Manuscript Revised: 7 NOV 2009
  6. Manuscript Received: 26 AUG 2009

Funded by

  • NSFC. Grant Numbers: 20607011, 20875055
  • NCET-06-0582,
  • the Cultivation Fund of the Key Scientific and Technical Innovation Project, Ministry of Education of China. Grant Number: 708058
  • Excellent Young Scientists and Key Science-Technology Project in Shandong Province. Grant Numbers: 2007BS08005, 2008GG10006012

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Keywords:

  • bovine insulin;
  • oxidative damage;
  • evaluation strategy;
  • cyclic voltammetry

Abstract

Research on protein oxidative damage may give insight into the nature of protein functions and pathological conditions. In this work, the oxidative damage of bovine insulin on Au electrode was investigated by cyclic voltammetry (CV). The experimental results show that there are two anodic peaks for the oxidative damage of bovine insulin, which arise from the oxidation of the exposed disulfide bond S[BOND]SCYS7A,CYS7B, forming sulfenic acid RSOH (1.20 V, vs. SCE), sulfinic acid RSO2H and sulfonic acid RSO3H (1.35 V, vs. SCE). These in vitro findings not only demonstrate the applicability of CV in simulating/evaluating the oxidative damage of nonredox proteins but also find two promising candidates (two anodic peaks) for measuring insulin.

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