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Mcl-1–Bim complexes accommodate surprising point mutations via minor structural changes
Article first published online: 11 JAN 2010
DOI: 10.1002/pro.329
Copyright © 2010 The Protein Society
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How to Cite
Fire, E., Gullá, S. V., Grant, R. A. and Keating, A. E. (2010), Mcl-1–Bim complexes accommodate surprising point mutations via minor structural changes. Protein Science, 19: 507–519. doi: 10.1002/pro.329
Publication History
- Issue published online: 22 FEB 2010
- Article first published online: 11 JAN 2010
- Accepted manuscript online: 11 JAN 2010 12:00AM EST
- Manuscript Accepted: 15 DEC 2009
- Manuscript Revised: 11 DEC 2009
- Manuscript Received: 27 OCT 2009
Funded by
- NIH. Grant Numbers: GM084181, P50-GM068762
References
- 1( 2003) The Bcl-2 protein family: sensors and checkpoints for life-or-death decisions. Mol Immunol 39: 615–647.
- 2, ( 2008) BH3-only proteins in apoptosis and beyond: an overview. Oncogene 27( Suppl 1): S2–S19.
- 3, , , , , ( 2002) Distinct BH3 domains either sensitize or activate mitochondrial apoptosis, serving as prototype cancer therapeutics. Cancer Cell 2: 183–192.
- 4, , , , , , , ( 2005) Proapoptotic Bak is sequestered by Mcl-1 and Bcl-xL, but not Bcl-2, until displaced by BH3-only proteins. Genes Dev 19: 1294–1305.
- 5, , , , , , , , , , , ( 1997) Structure of Bcl-xL-Bak peptide complex: recognition between regulators of apoptosis. Science 275: 983–986.
- 6, , , , , , , , ( 2007) Structural insights into the degradation of Mcl-1 induced by BH3 domains. Proc Natl Acad Sci USA 104: 6217–6222.
- 7, , , ( 2008) Structural plasticity underpins promiscuous binding of the prosurvival protein A1. Structure 16: 818–829.
- 8, , , , , ( 2006) Structural model of the BCL-w-BID peptide complex and its interactions with phospholipid micelles. Biochemistry 45: 2250–2256.
- 9, , , , , , , , ( 2001) Solution structure of the antiapoptotic protein bcl-2. Proc Natl Acad Sci USA 98: 3012–3017.
- 10, , , , , , ( 2005) BH3 domains of BH3-only proteins differentially regulate Bax-mediated mitochondrial membrane permeabilization both directly and indirectly. Mol Cell 17: 525–535.
- 11, , , , , , , , , , ( 2000) Rationale for Bcl-xL/Bad peptide complex formation from structure, mutagenesis, and biophysical studies. Protein Sci 9: 2528–2534.
- 12, , , , , , , , , ( 2005) Differential targeting of prosurvival Bcl-2 proteins by their BH3-only ligands allows complementary apoptotic function. Mol Cell 17: 393–403.
- 13, , , , , , ( 2006) Mitochondria primed by death signals determine cellular addiction to antiapoptotic BCL-2 family members. Cancer Cell 9: 351–365.
- 14, , , , , ( 2007) BH3 profiling identifies three distinct classes of apoptotic blocks to predict response to ABT-737 and conventional chemotherapeutic agents. Cancer Cell 12: 171–185.
- 15( 2006) Mcl-1: a highly regulated cell death and survival controller. J Biomed Sci 13: 201–204.
- 16, , , , , ( 2005) Solution structure of prosurvival Mcl-1 and characterization of its binding by proapoptotic BH3-only ligands. J Biol Chem 280: 4738–4744.
- 17, , , , ( 1993) MCL1, a gene expressed in programmed myeloid cell differentiation, has sequence similarity to BCL2. Proc Natl Acad Sci USA 90: 3516–3520.
- 18, ( 2000) The hallmarks of cancer. Cell 100: 57–70.
- 19, , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , ( 2005) An inhibitor of Bcl-2 family proteins induces regression of solid tumours. Nature 435: 677–681.
- 20, , , , , , , , , , , , , , , , , ( 2008) ABT-263: a potent and orally bioavailable Bcl-2 family inhibitor. Cancer Res 68: 3421–3428.
- 21, , , , , , , , , , , , , , , , , , , , , , , , , ( 2006) Mechanisms of apoptosis sensitivity and resistance to the BH3 mimetic ABT-737 in acute myeloid leukemia. Cancer Cell 10: 375–388.
- 22, , , , , , , , , , , , ( 2006) The BH3 mimetic ABT-737 targets selective Bcl-2 proteins and efficiently induces apoptosis via Bak/Bax if Mcl-1 is neutralized. Cancer Cell 10: 389–399.
- 23, , , , , , , , , , ( 2008) A novel BH3 ligand that selectively targets Mcl-1 reveals that apoptosis can proceed without Mcl-1 degradation. J Cell Biol 180: 341–355.
- 24, , , ( 2008) Hydrophile scanning as a complement to alanine scanning for exploring and manipulating protein-protein recognition: application to the Bim BH3 domain. Protein Sci 17: 1232–1240.
- 25, , , , , , ( 2007) Crystal structure of ABT-737 complexed with Bcl-xL: implications for selectivity of antagonists of the Bcl-2 family. Cell Death Differ 14: 1711–1713.
- 26, , , , , ( 2008) Structure of the BH3 domains from the p53-inducible BH3-only proteins Noxa and Puma in complex with Mcl-1. J Mol Biol 380: 958–971.
- 27, , , , , , , ( 2009) Conformational changes in BCL-2 pro-survival proteins determine their capacity to bind ligands. J Biol Chem 284: 30508–30517.
- 28, , , , , , , ( 2009) High-resolution structural characterization of a helical alpha/beta-peptide foldamer bound to the anti-apoptotic protein Bcl-xL. Angew Chem Int Ed Engl 48: 4318–4322.
- 29, , , ( 2007) Molecular basis of Bcl-xL's target recognition versatility revealed by the structure of Bcl-xL in complex with the BH3 domain of Beclin-1. J Mol Biol 372: 223–235.
- 30, , ( 2007) Crystal structure of the Bcl-XL-Beclin 1 peptide complex: Beclin 1 is a novel BH3-only protein. J Biol Chem 282: 13123–13132.
- 31, , , , ( 2003) The structure of a Bcl-xL/Bim fragment complex: implications for Bim function. Immunity 19: 341–352.
- 32, , , , , , , , , , , ( 1996) X-ray and NMR structure of human Bcl-xL, an inhibitor of programmed cell death. Nature 381: 335–341.
- 33, , ( 2007) Modeling backbone flexibility to achieve sequence diversity: the design of novel alpha-helical ligands for Bcl-xL. J Mol Biol 371: 1099–1117.
- 34, , , , , , , , ( 2008) Completing the family portrait of the anti-apoptotic Bcl-2 proteins: crystal structure of human Bfl-1 in complex with Bim. FEBS Lett 582: 3590–3594.
- 35, , , , ( 1993) Atomic structures of the human immunophilin FKBP-12 complexes with FK506 and rapamycin. J Mol Biol 229: 105–124.
- 36, ( 2002) Substructure solution with SHELXD. Acta Crystallogr D Biol Crystallogr 58: 1772–1779.
- 37, ( 1997) Maximum-likelihood heavy-atom parameter refinement for multiple isomorphous replacement and multiwavelength anomalous diffraction methods. Methods Enzymol 276: 472–494.
- 38, ( 2004) Coot: model-building tools for molecular graphics. Acta Crystallogr D Biol Crystallogr 60: 2126–2132.
- 39, , ( 1997) Refinement of macromolecular structures by the maximum-likelihood method. Acta Crystallogr D Biol Crystallogr 53: 240–255.
- 40, , , , , , , , , , ( 2007) MolProbity: all-atom contacts and structure validation for proteins and nucleic acids. Nucleic Acids Res 35: W375–W383.
- 41, ( 1997) Processing of x-ray diffraction data collected in oscillation mode. Methods Enzymol 276: 307–326.
- 42, , ( 2004) Likelihood-enhanced fast rotation functions. Acta Crystallogr D Biol Crystallogr 60: 432–438.
- 43, , , , , , , , , ( 2002) PHENIX: building new software for automated crystallographic structure determination. Acta Crystallogr D Biol Crystallogr 58: 1948–1954.

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