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Genetic selection system for improving recombinant membrane protein expression in E. coli

  1. Elizabeth Massey-Gendel1,
  2. Anni Zhao1,
  3. Gabriella Boulting1,†,
  4. Hye-Yeon Kim2,‡,
  5. Michael A. Balamotis3,
  6. Len M. Seligman4,
  7. Robert K. Nakamoto5,
  8. James U. Bowie1,2,*

Article first published online: 15 DEC 2008

DOI: 10.1002/pro.39

Issue

Protein Science

Protein Science

Volume 18, Issue 2, pages 372–383, February 2009

Additional Information

How to Cite

Massey-Gendel, E., Zhao, A., Boulting, G., Kim, H.-Y., Balamotis, M. A., Seligman, L. M., Nakamoto, R. K. and Bowie, J. U. (2009), Genetic selection system for improving recombinant membrane protein expression in E. coli. Protein Science, 18: 372–383. doi: 10.1002/pro.39

Author Information

  1. 1

    Department of Chemistry and Biochemistry, University of California, Los Angeles, California

  2. 2

    UCLA-DOE Institute for Genomics and Proteomics, University of California, Los Angeles, California

  3. 3

    Department of Microbiology, Immunology, and Molecular Genetics, University of California, Los Angeles, California

  4. 4

    Department of Biology, Pomona College, Claremont, California

  5. 5

    Department of Molecular Physiology & Biological Physics, University of Virginia, Charlottesville, Virginia

  1. †

    Department of Molecular and Cellular Biology, Harvard University, Cambridge, MA

  2. ‡

    Center for Magnetic Resonance Research, Korea Basic Science Institute, 113 Gwahangno, Yusung-gu, Daejeon, 305-333, Korea

*Boyer Hall, University of California, Los Angeles, 611 Charles E. Young Dr. E, Los Angeles, CA 90095-1570

Publication History

  1. Issue published online: 30 JAN 2009
  2. Article first published online: 15 DEC 2008
  3. Accepted manuscript online: 15 DEC 2008 12:00AM EST
  4. Manuscript Accepted: 12 NOV 2008
  5. Manuscript Revised: 11 NOV 2008
  6. Manuscript Received: 17 SEP 2008

Funded by

  • NIH Roadmap Grant. Grant Number: RO1 GM075922
  • NIH/NIGMS Ruth L. Kirschstein National Research Service Award. Grant Number: GM07185

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