Kuei-Min Chung and Jai-Hong Cheng contributed equally to this article.
Article
You have full text access to this OnlineOpen article
The dimeric transmembrane domain of prolyl dipeptidase DPP-IV contributes to its quaternary structure and enzymatic activities
Article first published online: 23 JUN 2010
DOI: 10.1002/pro.443
Copyright © 2010 The Protein Society
Additional Information
How to Cite
Chung, K.-M., Cheng, J.-H., Suen, C.-S., Huang, C.-H., Tsai, C.-H., Huang, L.-H., Chen, Y.-R., Wang, A. H.-J., Jiaang, W.-T., Hwang, M.-J. and Chen, X. (2010), The dimeric transmembrane domain of prolyl dipeptidase DPP-IV contributes to its quaternary structure and enzymatic activities. Protein Science, 19: 1627–1638. doi: 10.1002/pro.443
Publication History
- Issue published online: 23 AUG 2010
- Article first published online: 23 JUN 2010
- Manuscript Accepted: 16 JUN 2010
- Manuscript Revised: 13 JUN 2010
- Manuscript Received: 21 APR 2010
Funded by
- NRPGM
- National Science Council. Grant Numbers: NSC96-3112-B-400-004, NSC-95-3114-P-002-005-Y
- National Health Research Institutes, Taiwan, ROC
References
- 1, ( 2003) Prolyl peptidases: a serine protease subfamily with high potential for drug discovery. Curr Opin Chem Biol 7: 496–504.
- 2, ( 1988) Membrane orientation of rat gp110 as studied by in vitro translation. J Biol Chem 263: 16892–16898.
- 3, , , , , , , , , , ( 2007) Caveolin-1 triggers T-cell activation via CD26 in association with CARMA1. J Biol Chem 282: 10117–10131.
- 4, , , ( 1994) Golgi retardation in Madin-Darby canine kidney and Chinese hamster ovary cells of a transmembrane chimera of two surface proteins. J Biol Chem 269: 1985–1994.
- 5( 1991) Sequences within and adjacent to the transmembrane segment of alpha-2,6-sialyltransferase specify Golgi retention. EMBO J 10: 3577–3588.
- 6, ( 1990) Molecular dissection of the NH2-terminal signal/anchor sequence of rat dipeptidyl peptidase IV. J Cell Biol 111: 323–328.
- 7, , , , , , , , , ( 2003) The crystal structure of dipeptidyl peptidase IV (CD26) reveals its functional regulation and enzymatic mechanism. Proc Natl Acad Sci USA 100: 5063–5068.
- 8, , , ( 2003) Crystal structure of human dipeptidyl peptidase IV/CD26 in complex with a substrate analog. Nat Struct Biol 10: 19–25.
- 9, , , , , ( 2003) Structural basis of proline-specific exopeptidase activity as observed in human dipeptidyl peptidase-IV. Structure 11: 947–959.
- 10, , ( 1998) Prolyl oligopeptidase: an unusual beta-propeller domain regulates proteolysis. Cell 94: 161–170.
- 11, , , , , , , ( 2004) One site mutation disrupts dimer formation in human DPP-IV proteins. J Biol Chem 279: 52338–52345.
- 12, , , , ( 2006) Identification of hydrophobic residues critical for DPP-IV dimerization. Biochemistry 45: 7006–7012.
- 13, , , ( 2000) Roles of cysteines in rat dipeptidyl peptidase IV/CD26 in processing and proteolytic activity. Eur J Biochem 267: 5093–5100.Direct Link:
- 14( 1994) Dimeric assembly of enterocyte brush border enzymes. Biochemistry 33: 1599–1605.
- 15, , , , , , ( 2001) TM Finder: a prediction program for transmembrane protein segments using a combination of hydrophobicity and nonpolar phase helicity scales. Protein Sci 10: 212–219.Direct Link:
- 16, , ( 2006) Evidence for assembly of small multidrug resistance proteins by a “two-faced” transmembrane helix. J Biol Chem 281: 15546–15553.
- 17, , ( 1999) A bioluminescence resonance energy transfer (BRET) system: application to interacting circadian clock proteins. Proc Natl Acad Sci USA 96: 151–156.
- 18, ( 2006) Illuminating insights into protein-protein interactions using bioluminescence resonance energy transfer (BRET). Nat Methods 3: 165–174.
- 19, , ( 1991) Predicting coiled coils from protein sequences. Science 252: 1162–1164.
- 20, , , ( 2006) Paircoil2: improved prediction of coiled coils from sequence. Bioinformatics 22: 356–358.
- 21, , , , ( 1992) Sequence specificity in the dimerization of transmembrane alpha-helices. Biochemistry 31: 12719–12725.
- 22, , ( 1997) A transmembrane helix dimer: structure and implications. Science 276: 131–133.
- 23, , , , ( 2000) Modulation of glycophorin A transmembrane helix interactions by lipid bilayers: molecular dynamics calculations. J Mol Biol 302: 727–746.
- 24, , , ( 1999) A heptad motif of leucine residues found in membrane proteins can drive self-assembly of artificial transmembrane segments. J Biol Chem 274: 9265–9270.
- 25, ( 2001) In vitro selection of membrane-spanning leucine zipper protein-protein interaction motifs using POSSYCCAT. J Biol Chem 276: 45580–45587.
- 26, , , , ( 2006) A transmembrane leucine zipper is required for activation of the dimeric receptor tyrosine kinase DDR1. J Biol Chem 281: 22744–22751.
- 27, , ( 2004) Folding of helical membrane proteins: the role of polar, GxxxG-like and proline motifs. Curr Opin Struct Biol 14: 465–479.
- 28, , ( 1999) A turn propensity scale for transmembrane helices. J Mol Biol 288: 141–145.
- 29, ( 1978) Empirical predictions of protein conformation. Annu Rev Biochem 47: 251–276.
- 30, ( 1990) A thermodynamic scale for the helix-forming tendencies of the commonly occurring amino acids. Science 250: 646–651.
- 31, , , , ( 2000) Interhelical hydrogen bonding drives strong interactions in membrane proteins. Nat Struct Biol 7: 154–160.
- 32, , , ( 2000) Asparagine-mediated self-association of a model transmembrane helix. Nat Struct Biol 7: 161–166.
- 33, , ( 2002) Motifs of serine and threonine can drive association of transmembrane helices. J Mol Biol 316: 799–805.
- 34, , ( 2001) Polar side chains drive the association of model transmembrane peptides. Proc Natl Acad Sci USA 98: 880–885.
- 35, , , , , , , , ( 2001) The TXP motif in the second transmembrane helix of CCR5. A structural determinant of chemokine-induced activation. J Biol Chem 276: 13217–13225.
- 36, , ( 2002) Proline-induced distortions of transmembrane helices. J Mol Biol 323: 951–960.
- 37, , ( 1996) Improved prediction for the structure of the dimeric transmembrane domain of glycophorin A obtained through global searching. Proteins 26: 257–261.Direct Link:
- 38, , ( 1997) The effect of point mutations on the free energy of transmembrane alpha-helix dimerization. J Mol Biol 272: 266–275.
- 39( 2005) Solving the membrane protein folding problem. Nature 438: 581–589.
- 40, , , ( 2009) Polar residues in transmembrane helices can decrease electrophoretic mobility in polyacrylamide gels without causing helix dimerization. Biochim Biophys Acta 1788: 1321–1331.
- 41, , , ( 2007) Protein folding by zipping and assembly. Proc Natl Acad Sci USA 104: 11987–11992.
- 42, , , , , , , ( 2001) Human ITCH is a coregulator of the hematopoietic transcription factor NF-E2. Genomics 73: 238–241.
- 43, ( 2006) Quantitative analysis of muscarinic acetylcholine receptor homo- and heterodimerization in live cells: regulation of receptor down-regulation by heterodimerization. J Biol Chem 281: 5416–5425.
- 44, , ( 2005) PDB_TM: selection and membrane localization of transmembrane proteins in the protein data bank. Nucleic Acids Res 33: D275–D278.
- 45( 1991) MOLSCRIPT: a program to produce both detailed and schematic plots of protein structures. J Appl Cryst 24: 946–950.Direct Link:
- 46, ( 1997) Raster3D photorealistic molecular graphics. Methods Enzymol 277: 505–524.

1469-896X/asset/olbannerleft.gif?v=1&s=d218899ae53b2862ab119790ed504b8d72122fb3)
1469-896X/asset/olbannerright.gif?v=1&s=59470eb9a1d9b7b13b1be75e9445e6c46ee2214f)
