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High-level expression and purification of Cys-loop ligand-gated ion channels in a tetracycline-inducible stable mammalian cell line: GABAA and serotonin receptors

  1. Zuzana Dostalova1,
  2. Aiping Liu1,
  3. Xiaojuan Zhou1,
  4. Sarah L. Farmer1,
  5. Eileen S. Krenzel1,†,
  6. Enrique Arevalo1,‡,
  7. Rooma Desai1,
  8. Paula L. Feinberg-Zadek1,§,
  9. Paul A. Davies1,¶,
  10. Innocent H. Yamodo1,
  11. Stuart A. Forman1,
  12. Keith W. Miller1,2,*

Article first published online: 26 JUL 2010

DOI: 10.1002/pro.456

Issue

Protein Science

Protein Science

Volume 19, Issue 9, pages 1728–1738, September 2010

Additional Information

How to Cite

Dostalova, Z., Liu, A., Zhou, X., Farmer, S. L., Krenzel, E. S., Arevalo, E., Desai, R., Feinberg-Zadek, P. L., Davies, P. A., Yamodo, I. H., Forman, S. A. and Miller, K. W. (2010), High-level expression and purification of Cys-loop ligand-gated ion channels in a tetracycline-inducible stable mammalian cell line: GABAA and serotonin receptors. Protein Science, 19: 1728–1738. doi: 10.1002/pro.456

Author Information

  1. 1

    Department of Anesthesia and Critical Care, Massachusetts General Hospital, Harvard Medical School, Boston, Massachusetts 02114

  2. 2

    Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, Boston, Massachusetts 02115

  1. †

    Boston University School of Medicine, Department of Physiology and Biophysics, 700 Albany Street, Boston, MA 02118

  2. ‡

    Momenta Pharmaceuticals, 675 West Kendall Street, Cambridge, MA 02145

  3. §

    McGovern Institute for Brain Research, Massachusetts Institute of Technology, 77 Massachusetts Ave., Cambridge, MA 02139

  4. Tufts University School of Medicine, Department of Neuroscience, 136 Harrison Ave, Boston, MA 02111

*Department of Anesthesia and Critical Care, Massachusetts General Hospital, 32 Fruit Street, Boston, MA 02114

Publication History

  1. Issue published online: 23 AUG 2010
  2. Article first published online: 26 JUL 2010
  3. Manuscript Accepted: 6 JUL 2010
  4. Manuscript Revised: 24 JUN 2010
  5. Manuscript Received: 8 MAY 2010

Funded by

  • National Institute of General Medical Sciences. Grant Number: GM 58448
  • Department of Anesthesia, Critical Care and Pain Medicine, Massachusetts General Hospital

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