On the role of surface tension in the stabilization of globular proteins

Authors

  • Tiao-Yin Lin,

    1. Graduate Department of Biochemistry, Brandeis University, Waltham, Massachusetts 02254
    Current affiliation:
    1. Institute of Biological Science and Technology, National Chiao Tung University, 75 Poai Street, Hsinchu, Taiwan 300, Republic of China
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  • Serge N. Timasheff

    Corresponding author
    1. Graduate Department of Biochemistry, Brandeis University, Waltham, Massachusetts 02254
    • Graduate Department of Biochemistry, Brandeis University, 415 South Street, Waltham, Massachusetts 02254–9110
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Abstract

The stabilization of proteins by a variety of co-solvents can be related to their property of increasing the surface tension of water. It is demonstrated that, during the thermal unfolding of proteins, this increase of the surface tension can be overcome by the increase in the temperature of the solution at the midpoint of the transition, Tm, and the weak binding of co-solvent molecules. Three such co-solvents were studied: trehalose, lysine hydrochloride (LysHCl), and arginine hydrochloride (ArgHCl). Trehalose and LysHCl increase the midpoint of Tm. The increase of the surface tension by addition of trehalose is completely compensated by its decrease due to the increase in Tm. However, for LysHCl, the increase of the surface tension by the co-solvent is partly reduced by its binding to the protein. For trehalose, preferential interaction measurements with RNaseA demonstrate that it is totally excluded from the protein. In contrast, LysHCl gives evidence of binding to RNaseA. ArgHCl also increases the surface tension of water. Nevertheless, Tm of RNaseA decreases on addition of ArgHCl to the solution. Preferential interaction measurements showed very small values of preferential hydration of the native protein, indicating extensive binding of ArgHCl to the protein. During unfolding, the amount of additional ArgHCl binding is sufficiently large to counteract the surface tension effects, and the protein is destabilized. Therefore, although surface tension appears to be a critical factor in the stabilization of proteins, its increase by co-solvent does not ensure increased stabilization. The binding of ligands can reduce significantly, or even overwhelm, its effects.

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