Accelerated Communication
You have full text access to this OnlineOpen article
T-shaped arrangement of the recombinant agrin G3 – IgG Fc protein
Article first published online: 26 APR 2011
DOI: 10.1002/pro.628
Copyright © 2011 The Protein Society
Additional Information
How to Cite
Patel, T. R., Meier, M., Li, J., Morris, G., Rowe, A. J. and Stetefeld, J. (2011), T-shaped arrangement of the recombinant agrin G3 – IgG Fc protein. Protein Science, 20: 931–940. doi: 10.1002/pro.628
Publication History
- Issue published online: 17 MAY 2011
- Article first published online: 26 APR 2011
- Accepted manuscript online: 29 MAR 2011 08:33AM EST
- Manuscript Accepted: 16 MAR 2011
- Manuscript Revised: 14 MAR 2011
- Manuscript Received: 7 FEB 2011
Funded by
- CIHR-Regional Partnership Program and the Manitoba Institute for Child Health
References
- 1, , , ( 1995) Agrin is a heparan sulfate proteoglycan. J Biol Chem 270: 3392–3399.
- 2, ( 1995) The role of agrin in synapse formation. Ann Rev Neuro 18: 443–462.
- 3, ( 1989) Agrin-related molecules are concentrated at acetylcholine receptor clusters in normal and aneural developing muscle. J Cell Biol 108: 1527–1535.
- 4, , , , , , ( 1987) Identification of agrin, a synaptic organizing protein from Torpedo electric organ. J Cell Biol 105: 2471–2478.
- 5( 1989) Agrin-induced specializations contain cytoplasmic, membrane, and extracellular matrix-associated components of the postsynaptic apparatus. J Neurosci 9: 1294–1302.
- 6, , , , ( 2001) Physiological regulation of the immunological synapse by agrin. Science 292: 1681–1686.
- 7, , , , ( 2006) α3Na+/K+-ATPase is a neuronal receptor for agrin. Cell 125: 359–369.
- 8, , ( 2003) The COOH-terminal domain of agrin signals via a synaptic receptor in central nervous system neurons. J Cell Biol 161: 923–932.
- 9, , , , ( 2005) HIV-1-infected blood mononuclear cells form an integrin- and agrin-dependent viral synapse to induce efficient HIV-1 transcytosis across epithelial cell monolayer. Mol Biol Cell 16: 4267–4279.
- 10, , , , , , , , ( 2008) Lrp4 is a receptor for agrin and forms a complex with MuSK. Cell 135: 334–342.
- 11, , , , , ( 2008) LRP4 serves as a coreceptor of agrin. Neuron 60: 285–297.
- 12, , , , , , ( 1996) Defective neuromuscular synaptogenesis in agrin-deficient mutant mice. Cell 85: 525–535.
- 13, , , , , ( 2001) Distinct roles of nerve and muscle in postsynaptic differentiation of the neuromuscular synapse. Nature 410: 1057–1064.
- 14, , , , , , , , ( 2001) The laminin-binding domain of agrin is structurally related to N-TIMP-1. Nat Struct Biol 8: 705–709.
- 15, , , , , ( 2005) Structure and laminin-binding specificity of the NtA domain expressed in eukaryotic cells. Matrix Biol 23: 507–513.
- 16, , , , , ( 2003) Mapping of the laminin-binding site of the N-terminal agrin domain (NtA). EMBO J 22: 529–536.
- 17, ( 2009) An interdomain disulfide bridge links the NtA and first FS domain in agrin. Protein Sci 18: 2421–2428.
- 18, , , , , , , , , ( 2004) Modulation of agrin function by alternative splicing and Ca2+ binding. Structure 12: 503–515.
- 19, , , , , , , ( 2006) Activation of muscle-specific receptor tyrosine kinase and binding to dystroglycan are regulated by alternative mRNA splicing of agrin. J Biol Chem 281: 36835–36845.
- 20, , , , , ( 1996) Alternative splicing of agrin alters its binding to heparin, dystroglycan, and the putative agrin receptor. Neuron 16: 755–767.
- 21, , ( 1996) Alternative RNA splicing that determines agrin activity regulates binding to heparin and alpha-dystroglycan. Development 122: 1663–1672.
- 22, , , , ( 1993) The ability of agrin to cluster AChRs depends on alternative splicing and on cell surface proteoglycans. Neuron 11: 491–502.
- 23, , ( 1995) Acetylcholine receptor-aggregating activity of agrin isoforms and mapping of the active site. J Cell Biol 128: 625–636.
- 24, ( 1996) Agrin binding to alpha-dystroglycan. J Biol Chem 271: 5231–5236.
- 25, , , , , ( 1996) Alternative splicing of agrin regulates its binding to heparin, alpha -dystroglycan, and the cell surface. Proc Natl Acad Sci USA 93: 7369–7374.
- 26, , ( 1999) Identification of extracellular matrix ligands for the heparan sulfate proteoglycan agrin. Exp Cell Res 249: 54–64.
- 27, ( 2010) New role for Agrin in T cells and its potential importance in immune system regulation. Arthritis Res Therapy 12: 205–211.
- 28, , , , ( 2006) α3Na+/K+-ATPase is a neuronal receptor for agrin. Cell 125: 359–369.
- 29, , ( 2010) How are ion pumps and agrin signaling integrated? Trends Biochem Sci 35: 653–659.
- 30( 2003) Surface plasmon resonance as a probe of protein isomerization. Anal Biochem 318: 1–12.
- 31( 2004) SPR for molecular interaction analysis: a review of emerging application areas. J Mol Recog 17: 151–161.
- 32, , , , , , , ( 2010) Nano-structure of the laminin γ-1 short arm reveals an extended and curved multidomain assembly. Matrix Biol 29: 565–572.
- 33, , , , Computer-aided interpretation of analytical sedimentation data for proteins. In: HardingSE, RoweAJ, HortonJC, Eds. ( 1992) Analytical ultracentrifugation in biochemistry and polymer science. Cambridge, United Kingdom: Royal Society of Chemistry, pp 90–125.
- 34, , ( 2010) Global structure of HIV-1 neutralizing antibody IgG1 b12 is asymmetric. Biochem Biophys Res Comm 391: 947–951.
- 35, , , The ELLIPS suit of whole-body protein conformation algorithms for Microsoft Windowns. In: ScootDJ, HardingSE, RoweAJ, Eds. ( 2005) Analytical ultracentrifugation techniques and methoda. Cambdrige: Royal Society of Chemistry, pp 468–483.
- 36, , , , , , , ( 2001) Crystallohydrodynamics for solving the hydration problem for multi-domain proteins: open physiological conformations for human IgG. Biophys Chem 93: 181–196.
- 37( 1961) Physical chemistry of macromolecules, New York: Wiley.
- 38( 2000) Size distribution analysis of macromolecules by sedimentation velocity ultracentrifugation and Lamm equation modeling. Biophys J 78: 1606–1619.
- 39, , ( 2008) Characterizing protein-protein interactions by sedimentation velocity analytical ultracentrifugation. Curr Protoc Immunol, Chapter 18, Unit 18. 15.
- 40( 1985) Physical biochemistry, Englewood Cliffs, NJ: Prentice Hall.
- 41, , , ( 2006) ATSAS 2.1, a program package for small-angle scattering data analysis. J Appl Cryst 39: 277–286.
- 42( 1992) Static and dynamic light scattering approaches to structure determination of biopolymers. In: HardingSE, SattelleDB, BloomfieldVA, Eds. Laser light scattering in biochemistry (.). Cambdrige: Royal Society of Chemistry. pp 3–22.
- 43, , , ( 1999) The Fab and Fc fragments of IgA1 exhibit a different arrangement from that in IgG: a study by X-ray and neutron solution scattering and homology modelling. J Mol Biol 286: 1421–1447.
- 44, , Calculating sedimentation coefficient distributions by direct modeling of sedimentation velocity concentration profiles. Methods in Enzymology. In: BrandL, JohnsonML. Eds. ( 2004) Numerical computer methods, Part E Vol. 3 84 edit. Academic Press. pp 185–212.
- 45( 1998) Sedimentation analysis of noninteracting and self-associating solutes using numerical solutions to the Lamm equation. Biophys J 75: 1503–1512.
- 46, , , , ( 2003) PRIMUS: a Windows PC-based system for small-angle scattering data analysis. J Appl Cryst 36: 1277–1282.
- 47, , , , , , , ( 2010) Nano-structure of the laminin gamma-1 short arm reveals an extended and curved multidomain assembly. Matrix Biol 29: 565–572.
- 48( 1992) Determination of the regularization parameter in indirect-transform methods using perceptual criteria. J Appl Cryst 25: 495–503.
- 49, ( 1955) Small angle scattering of X-rays, New York: Wiley.
- 50, , ( 1995) CRYSOL - a program to evaluate X-ray solution scattering of biological macromolecules from attomic coordinates. J Appl Cryst 28: 768–773.
- 51, ( 2005) Global rigid body modeling of macromolecular complexes against small-angle scattering data. Biophys J 89: 1237–1250.
- 52, ( 2003) ModLoop: automated modeling of loops in protein structures. Bioinformatics 19: 2500–2501.
- 53, ( 2003) Uniqueness of ab initio shape determination in small-angle scattering. J Appl Cryst 36: 860–864.
- 54, ( 2001) Automated matching of high- and low-resolution structural models. J Appl Cryst 34: 33–41.
- 55, , , , , , , , , , , , , ( 1998) Crystallography & NMR system: a new software suite for macromolecular structure determination. Acta Cryst D54: 905–921.
- 56

1469-896X/asset/olbannerleft.gif?v=1&s=d218899ae53b2862ab119790ed504b8d72122fb3)
1469-896X/asset/olbannerright.gif?v=1&s=59470eb9a1d9b7b13b1be75e9445e6c46ee2214f)
