NMR structure of the Bordetella bronchiseptica protein NP_888769.1 establishes a new phage-related protein family PF13554

Authors

  • Atia-tul- Wahab,

    1. Joint Center for Structural Genomics (http://www.jcsg.org), The Scripps Research Institute, La Jolla, California 92037
    2. Department of Molecular Biology, The Scripps Research Institute, La Jolla, California 92037
    Search for more papers by this author
    • Atia-tul-Wahab and Pedro Serrano contributed equally to this work. Atia-tul-Wahab's current address is Dr. Panjwani center for Molecular Medicine and Drug Research, International Center for Chemical and Biological Sciences, University of Karachi, Karachi-75270, Pakistan

  • Pedro Serrano,

    1. Joint Center for Structural Genomics (http://www.jcsg.org), The Scripps Research Institute, La Jolla, California 92037
    2. Department of Molecular Biology, The Scripps Research Institute, La Jolla, California 92037
    Search for more papers by this author
    • Atia-tul-Wahab and Pedro Serrano contributed equally to this work. Atia-tul-Wahab's current address is Dr. Panjwani center for Molecular Medicine and Drug Research, International Center for Chemical and Biological Sciences, University of Karachi, Karachi-75270, Pakistan

  • Michael Geralt,

    1. Joint Center for Structural Genomics (http://www.jcsg.org), The Scripps Research Institute, La Jolla, California 92037
    2. Department of Molecular Biology, The Scripps Research Institute, La Jolla, California 92037
    Search for more papers by this author
  • Kurt Wüthrich

    Corresponding author
    1. Joint Center for Structural Genomics (http://www.jcsg.org), The Scripps Research Institute, La Jolla, California 92037
    2. Department of Molecular Biology, The Scripps Research Institute, La Jolla, California 92037
    3. Skaggs Institute of Chemical Biology, The Scripps Research Institute, La Jolla, California 92037
    • Joint Center for Structural Genomics, The Scripps Research Institute, La Jolla, CA 92037
    Search for more papers by this author

Abstract

The solution structure of the hypothetical phage-related protein NP_888769.1 from the Gram-negative bacterium Bordetella bronchoseptica contains a well-structured core comprising a five-stranded, antiparallel β-sheet packed on one side against two α-helices and a short β-hairpin with three flexibly disordered loops extending from the central β-sheet. A homology search with the software DALI identified two Protein Data Bank deposits with Z-scores > 8, where both of these proteins have less than 8% sequence identity relative to NP_888769.1, and one has been functionally annotated as a lambda phage tail terminator protein. A sequence-homology analysis then confirmed that NP_888769.1 represents the first three-dimensional structural representative of a new protein family that was previously predicted by the Joint Center for Structural Genomics, which includes so far about 20 prophage proteins encoded in bacterial genomes.

Ancillary