Green fluorescent protein: A perspective

Authors

  • S. James Remington

    Corresponding author
    1. Institute of Molecular Biology and Department of Physics, University of Oregon, Eugene, Oregon 97403–1229
    • Institute of Molecular Biology and Department of Physics, University of Oregon, Eugene, OR 97403–1229
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Abstract

A brief personal perspective is provided for green fluorescent protein (GFP), covering the period 1994–2011. The topics discussed are primarily those in which my research group has made a contribution and include structure and function of the GFP polypeptide, the mechanism of fluorescence emission, excited state protein transfer, the design of ratiometric fluorescent protein biosensors and an overview of the fluorescent proteins derived from coral reef animals. Structure-function relationships in photoswitchable fluorescent proteins and nonfluorescent chromoproteins are also briefly covered.

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