• 1
    Simmons G, Reeves J, Rennekamp A, Amberg S, Piefer A, Bates P ( 2004) Characterization of severe acute respiratory syndrome-associated coronavirus (SARS CoV) spike glycoprotein-mediated viral entry. Proc Natl Acad Sci USA 101: 42404245.
  • 2
    Li W, Moore MJ, Vasilieva N, Sui J, Wong SK, Berne MA, Somasundaran M, Sullivan JL, Luzuriaga K, Greenough TC, Choe H, Farzan M ( 2003) Angiotensin-converting enzyme 2 is a functional receptor for the SARS coronavirus. Nature 426: 450454.
  • 3
    Liu S, Xiao G, Chen Y, He Y, Niu J, Escalente C, Xiong H, Farmar J, Debnath A, Tien P, Jiang S ( 2004) Interaction between heptad repeat 1 and 2 regions in spike protein of SARS-associated coronavirus: implications for virus fusogenic mechanism and identification effusion inhibitors. Lancet 363: 938947.
  • 4
    Tripet B, Howard M, Jobling M, Holmes R, Holmes K, Hodges R ( 2004) Structural characterization of the SARS-coronavirus spike S fusion protein core. J Biol Chem 279: 2083620849.
  • 5
    Simmons G, Gosalia D, Rennekamp A, Reeves J, Diamond S, Bates P ( 2005) Inhibitors of cathespin L prevent severe acute respiratory syndrome coronavirus entry. Proc Natl Acad Sci USA 102: 1187611881.
  • 6
    Caffrey M ( 2011) HIV envelope: challenges and opportunities for the discovery of entry inhibitors. Trends Microbiol 9: 191197.
  • 7
    Matthews T, Salgo M, Greenberg M, Chung J, DeMasi R, Bolognesi D ( 2004) Enfuvirtide: the first therapy to inhibit the entry of HIV-1 into host CD4 lymphocytes. Nat Rev Drug Discov 3: 215225.
  • 8
    Bosch B, Martina B, Van Der Zee R, Lepault J, Haijema B, Versluis C, Heck A, De Groot R, Osterhaus A, Rottier P ( 2004) Severe acute respiratory syndrome coronavirus (SARS-CoV) infection inhibition using spike protein heptad repeat-derived peptides. Proc Natl Acad Sci USA 101: 84558460.
  • 9
    Guo Y, Tisoncik J, McReynolds S, Lou G, Martinez O, Farzan M, Prabhakar B, Gallagher T, Rong L, Caffrey M ( 2009) Identification of a small region of SARS-CoV S protein critical for viral entry. J Mol Biol 394: 600605.
  • 10
    Supekar V, Bruckmann C, Ingallinella P, Bianchi E, Pessi A, Carfi A ( 2004) Structure of a proteolytically resistant core from the severe acute respiratory syndrome coronavirus S2 fusion protein. Proc Natl Acad Sci USA 101: 1795817963.
  • 11
    Xu Y, Lou Z, Liu Y, Pang H, Tien P, Gao G, Rao Z ( 2004) Crystal structure of the severe acute syndrome coronavirus spike protein fusion core. J Biol Chem 279: 4941449419.
  • 12
    Duquerroy S, Vigouroux A, Rottier P, Rey F, Bosch B ( 2005) Central ions an lateral asparagine/glutamine zippers stabilize the post-fusion hairpin conformation of the SARS coronavirus spike glycoprotein. Virology 335: 276285.
  • 13
    Deng Y, Liu J, Zheng Q, Yong W, Lu M ( 2006) Structures and polymorhic interactions of two heptad-repeat regions of the SARS virus S2 protein. Structure 14: 889899.
  • 14
    Hakansson-McReynolds S, Jiang S, Rong L, Caffrey M ( 2006) The solution structure of the SARS coronavirus heptad repeat 2 in the prefusion state. J Biol Chem 281: 1196511971.
  • 15
    McReynolds S, Jiang S, Ying G, Celigoy J, Schar C, Rong L, Caffrey M ( 2008) Characterization of the prefusion and transition states of Severe Acute Respiratory Syndrome Coronavirus S2-HR2. Biochemistry 47: 68026808.
  • 16
    McReynolds S, Jiang S, Rong L, Caffrey M ( 2009) Dynamics of the SARS coronavirus prefusion and transition states. J Magn Reson 201: 218221.
  • 17
    Buck M ( 1998) Trifluoroethanol and colleagues: cosolvents come of age. Recent studies with peptides and proteins. Q Rev Biophys 31: 297355.
  • 18
    Battiste JL, Wagner G ( 2000) Utilization of site-directed spin labeling and high-resolution heteronuclear nuclear magnetic resonance for global fold determination of large proteins with limited nuclear overhauser effect data. Biochemistry 39: 53555365.
  • 19
    Park S, Caffrey M, Johnson M, Fung L ( 2003) Solution structural studies on human erythrocyte alpha spectrin N-terminal tetramerization domain. J Biol Chem 278: 2183721844.
  • 20
    Celigoy J, McReynolds S, Caffrey M ( 2011) The SARS-CoV heptad repeat 2 exhibits pH-induced helix formation. Biochem Biophys Res Commun 412: 483486.
  • 21
    Huth J, Bewley C, Jackson B, Hinnebusch A, Clore G, Gronenborn A ( 1997) Design of an expression system for detecting folded protein domains and mapping macromolecular interactions by NMR. Protein Sci 6: 23592364.
  • 22
    Delaglio F, Grzesiek S, Vuister GW, Zhu G, Pfeifer J, Bax A ( 1995) NMRPipe: a multidimensional spectral processing system based on UNIX pipes. J Biomol NMR 6: 277293.
  • 23
    Jiang S, Jacobs A, Laue T, Caffrey M ( 2004) Solution structure of the Coxsackievirus and Adenovirus receptor domain 1. Biochemistry 43: 18471853.
  • 24
    Stein EG, Rice LM, Brunger AT ( 1997) Torsion-angle molecular dynamics as a new efficient tool for NMR structure calculation. J Magn Reson 124: 154164.
  • 25
    Nilges M, Clore GM, Gronenborn AM ( 1988) Determination of three-dimensional structures of proteins from interproton distance data by dynamical simulated annealing from a random array of atoms. Circumventing problems associated with folding. FEBS Lett 229: 317324.
  • 26
    Brunger A, Adams P, Clore G, DeLano W, Gros P, Grosse-Kunstleve R, Jiang J-S, Kuszewski J, Nilges N, Pannu N, Read R, Rice L, Simonson T, Warren G ( 1998) Crystallography and NMR system CNS: a new software system for macromolecular structure determination. Acta Cryst D54: 905921.
  • 27
    Kuszewski J, Gronenborn AM, Clore GM ( 1996) Improving the quality of NMR and crystallographic protein structures by means of a conformational database potential derived from structure databases. Protein Sci 5: 10671080.
  • 28
    Koradi R, Billeter, M, Wuthrich, K ( 1996) MOLMOL: a program for display and analysis of macromolecular structures. J Mol Graph 14: 2932.