1775 Quantitative in vivo solubility and reconstitution of truncated circular permutants of green fluorescent protein
Yao-Ming Huang, Sasmita Nayak, Christopher Bystroff
Green fluorescent protein can be split into two parts that sometimes reassemble to reconstitute fluorescence. Here, Huang, Nayak, and Bystroff exhaustively removed each of the 11 beta strands and the central helix from the GFP sequence and then measured the solubility in bacteria. By co-expressing each LOO-GFP with its left-out segment, the authors measured the ability of the LOO-GFPs to bind their missing piece and glow. What was found is that the solubility of the LOO-GFP depends very much on which part of the molecule was left out. Some parts of the protein are more essential to efficient folding than others.