A fluorescent fatty acid probe, DAUDA, selectively displaces two myristates bound in human serum albumin

Authors

  • Yu Wang,

    1. State Key Laboratory of Structural Chemistry, Fujian Institute of Research on the Structure of Matter, Chinese Academy of Sciences, Fuzhou, China
    2. Graduate School, Chinese Academy of Sciences, Beijing, China
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    • Yu Wang and Zhipu Luo contributed equally to this work.

  • Zhipu Luo,

    1. State Key Laboratory of Structural Chemistry, Fujian Institute of Research on the Structure of Matter, Chinese Academy of Sciences, Fuzhou, China
    2. Graduate School, Chinese Academy of Sciences, Beijing, China
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    • Yu Wang and Zhipu Luo contributed equally to this work.

  • Xiaoli Shi,

    1. State Key Laboratory of Structural Chemistry, Fujian Institute of Research on the Structure of Matter, Chinese Academy of Sciences, Fuzhou, China
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  • Huan Wang,

    1. Zhejiang Hisun Pharmaceutical Co., Taizhou, China
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  • Lei Nie,

    1. Zhejiang Hisun Pharmaceutical Co., Taizhou, China
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  • Mingdong Huang

    Corresponding author
    1. State Key Laboratory of Structural Chemistry, Fujian Institute of Research on the Structure of Matter, Chinese Academy of Sciences, Fuzhou, China
    • State Key Lab of Structural Chemistry, Fujian Institute of Research on the Structure of Matter, Chinese Academy of Sciences, Fuzhou, China
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Abstract

11-(Dansylamino) undecanoic acid (DAUDA) is a dansyl-type fluorophore and has widely used as a probe to determine the binding site for human serum albumin (HSA). Here, we reported that structure of HSA-Myristate-DAUDA ternary complex and identified clearly the presence of two DAUDA molecules at fatty acid (FA) binding site 6 and 7 of HSA, thus showing these two sites are weak FA binding sites. This result also show that DAUDA is an appropriate probe for FA site 6 and 7 on HSA as previous studied, but not a good probe of FA binding site 1 that is likely bilirubin binding site on HSA.

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