Expression of androgen receptor coactivators in normal and cancer prostate tissues and cultured cell lines
Article first published online: 20 MAY 2003
Copyright © 2003 Wiley-Liss, Inc.
Volume 56, Issue 3, pages 192–200, 1 August 2003
How to Cite
Mestayer, C., Blanchère, M., Jaubert, F., Dufour, B. and Mowszowicz, I. (2003), Expression of androgen receptor coactivators in normal and cancer prostate tissues and cultured cell lines. Prostate, 56: 192–200. doi: 10.1002/pros.10229
- Issue published online: 20 MAY 2003
- Article first published online: 20 MAY 2003
- Manuscript Accepted: 9 DEC 2002
- Manuscript Received: 9 MAY 2002
- Association de Recherche contre le Cancer. Grant Number: ARC No. 5356
- androgen receptor;
- prostate cancer;
- prostate cells culture;
- hormonal regulation
In prostate cancer cell lines, androgen receptor (AR) coactivators modulate the transcriptional activity of AR. However, very little is known about their expression in normal prostate tissue and during progression to cancer.
AR and coactivators ARA54, ARA55, ARA70, and SRC1 RNA were analyzed by RT-PCR in normal and tumoral tissues of the same prostate, in prostate cell lines, and after hormonal treatments of prostate epithelial cells.
AR-coactivators were expressed in normal and tumoral tissues and in cultured prostate cells; only ARA55 expression was decreased in tumoral relative to normal tissue of all seven prostates analyzed. It was not expressed in LNCaP and DU145 cancer cells and low in PNT2 immortalized cells in which all coactivator's expression were down regulated by DHT and up regulated by E2. In addition, coactivator's expression was increased in hyperplastic relative to normal prostate fibroblasts.
ARA55 is both an AR coactivator and a focal adhesion protein (Hic-5). Its role in the progression of prostate carcinoma may therefore involve these two different functions. Its decrease in cancer tissue suggests that it plays a different role than that expected, namely, facilitate cell proliferation and therefore mobility and metastasis. Prostate 56: 192–200, 2003. © 2003 Wiley-Liss, Inc.