Prostate cell lines secrete IL-8 in response to mycoplasma hominis through Toll-like receptor 2-mediated mechanism

Authors


Abstract

Background

The roles of Toll-like receptor 2 (TLR2) and Mycoplasma in prostate inflammation remain unclear. We investigated whether Mycoplasma induced inflammatory cytokine secretion through TLR2-mediated mechanism in prostate cancer cell line.

Methods

Cell surface expression of TLR2 on PC-3 cells were examined by flowcytometry. PC-3 cells were stimulated with Mycoplasma hominis (M. hominis), and IL-8 secretion and NF-κB activation were examined.

Results

PC-3 cells expressed TLR2 mRNA and cell surface TLR2 protein. The membrane fraction of M. hominis induced IL-8 secretion from PC-3 cells and NF-κB activation in a concentration-dependent manner. Transient transfection of the dominant negative mutant TLR2P681H into PC-3 cells attenuated M. hominis-induced IL-8 secretion and NF-κB activation. Antibody against the extracellular TLR2 domain significantly suppressed M. homnis-induced IL-8 secretion from the prostate cell lines including PC-3, PrEC, and transformed myofibroblasts.

Conclusions

These results clearly demonstrate that the prostate cell line can secrete inflammatory cytokine in response to M. hominis through a TLR2-mediated mechanism. © 2005 Wiley-Liss, Inc.

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