Original Article

Energy balance alters dunning R3327-H prostate tumor architecture, androgen receptor expression, and nuclear morphometry in rats

  1. Zhiming Liao,
  2. Shihua Wang,
  3. Brandy S. Wiegers†,
  4. Steven K. Clinton*

Article first published online: 15 MAR 2006

DOI: 10.1002/pros.20406

Issue

The Prostate

The Prostate

Volume 66, Issue 9, pages 945–953, 15 June 2006

Additional Information

How to Cite

Liao, Z., Wang, S., Wiegers, B. S. and Clinton, S. K. (2006), Energy balance alters dunning R3327-H prostate tumor architecture, androgen receptor expression, and nuclear morphometry in rats. The Prostate, 66: 945–953. doi: 10.1002/pros.20406

Author Information

  1. Division of Hematology and Oncology, Department of Internal Medicine, The Ohio State University, Columbus, Ohio

  1. †

    Department of Mathematics, University of California, Davis, California 95616.

*A434 Starling Loving Hall, 320 West 10th Ave, The Ohio State University, Columbus, OH 43210.

Publication History

  1. Issue published online: 8 MAY 2006
  2. Article first published online: 15 MAR 2006
  3. Manuscript Accepted: 13 DEC 2005
  4. Manuscript Received: 7 NOV 2005

Funded by

  • Public Health Service
  • National Institutes of Health
  • National Cancer Institute. Grant Numbers: R01CA72482, R01CA101113-01A1
  • The Comprehensive Cancer Center, The Ohio State University. Grant Number: P30 CA16058
  • National Science Foundation, Division of Mathematical Sciences. Grant Number: 9988006

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Keywords:

  • prostate cancer;
  • energy restriction;
  • image analysis;
  • nuclear morphometry;
  • biomarkers

Abstract

BACKGROUND

We hypothesize that diet or energy restriction may alter prostate tumor architecture and nuclear morphometry in a manner predictive of indolent biology.

METHODS

Adult male rats bearing the Dunning R3327-H tumor were provided free-access to the AIN76 diet or 30% diet/energy restriction for 16 weeks. Tumor tissue was harvested and stained for biomarker evaluation by quantitative digital image analysis.

RESULTS

Compared to controls, tumors from restricted rats showed a contracted stromal compartment with an increased epithelial/glandular area, and a greater number of smaller and more homogeneous glands. In parallel, the nuclei in tumors harvested from diet-restricted rats were smaller in size, showed a lower proportion with androgen receptor (AR) staining, and reduced intensity of AR staining.

CONCLUSIONS

Our observations support the hypothesis that diet/energy restriction alters prostate tumor architecture, AR expression, and nuclear morphology consistent with a more differentiated and less aggressive phenotype. Prostate 66: 945–953, 2006. © 2006 Wiley-Liss, Inc.

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