Original Article

Molecular PCA3 diagnostics on prostatic fluid

  1. Martijn P.M.Q. van Gils1,
  2. Erik B. Cornel2,
  3. Daphne Hessels1,
  4. W. Pim Peelen1,
  5. J. Alfred Witjes1,
  6. Peter F.A. Mulders1,
  7. Harry G. Rittenhouse3,
  8. Jack A. Schalken1,*

Article first published online: 17 APR 2007

DOI: 10.1002/pros.20564

Issue

The Prostate

The Prostate

Volume 67, Issue 8, pages 881–887, 1 June 2007

Additional Information

How to Cite

van Gils, M. P., Cornel, E. B., Hessels, D., Peelen, W. P., Witjes, J. A., Mulders, P. F., Rittenhouse, H. G. and Schalken, J. A. (2007), Molecular PCA3 diagnostics on prostatic fluid. The Prostate, 67: 881–887. doi: 10.1002/pros.20564

Author Information

  1. 1

    Department of Urology, Radboud University Nijmegen Medical Centre, Nijmegen, The Netherlands

  2. 2

    Department of Urology, Twente Hospital Group (Location SMT), Hengelo, The Netherlands

  3. 3

    Gen-Probe Incorporated, San Diego, CA

*Department of Urology (Internal postal code 267), Radboud University Nijmegen Medical Centre, P.O. Box 9101, 6500 HB Nijmegen, The Netherlands.

Publication History

  1. Issue published online: 23 APR 2007
  2. Article first published online: 17 APR 2007
  3. Manuscript Accepted: 2 JAN 2007
  4. Manuscript Received: 8 NOV 2006

Funded by

  • Dutch Cancer Society. Grant Number: KWF/KUN 2004-3152
  • Sixth EU Framework Programme for Research and Technological Development. Grant Number: LSHC-CT-2004-503011

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Keywords:

  • prostate cancer antigen 3;
  • human;
  • prostatic neoplasms;
  • diagnosis;
  • prostatic fluid;
  • urine

Abstract

BACKGROUND

The PCA3 test on urine can improve specificity in prostate cancer (PCa) diagnosis and could prevent unnecessary prostate biopsies. In this study, we evaluated the PCA3 test on prostatic fluid and compared this with the PCA3 test on urine in a clinical research setting.

METHODS

Prostatic fluid and urine samples from 67 men were collected following digital rectal examination (DRE). The sediments were analyzed using the quantitative APTIMA® PCA3 test. The results were compared with prostate biopsy results.

RESULTS

Using a PCA3 score of 66 as a cut-off value, the test on prostatic fluid had 65% sensitivity for the detection of PCa, 82% specificity and a negative predictive value of 82%. At a cut-off value of 43, the test on urine had 61% sensitivity, 80% specificity and a negative predictive value of 80%.

CONCLUSIONS

The PCA3 test can be performed on both urine and prostatic fluid in the diagnosis of PCa with comparable results. Prostate 67: 881–887, 2007. © 2007 Wiley-Liss, Inc.

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