Immunological evaluation of neoadjuvant peptide vaccination before radical prostatectomy for patients with localized prostate cancer
Article first published online: 17 APR 2007
Copyright © 2007 Wiley-Liss, Inc.
Volume 67, Issue 9, pages 933–942, 15 June 2007
How to Cite
Noguchi, M., Yao, A., Harada, M., Nakashima, O., Komohara, Y., Yamada, S., Itoh, K. and Matsuoka, K. (2007), Immunological evaluation of neoadjuvant peptide vaccination before radical prostatectomy for patients with localized prostate cancer. Prostate, 67: 933–942. doi: 10.1002/pros.20572
- Issue published online: 23 APR 2007
- Article first published online: 17 APR 2007
- Manuscript Accepted: 23 JAN 2007
- Manuscript Received: 7 JUN 2006
- Japan Society for the Promotion of Science. Grant Number: 15591721
- prostate cancer;
- cancer vaccine;
- T cell
The purpose of this study was to determine the safety and immune responses of pre-operative personalized peptide vaccine for patients with localized prostate cancer.
Ten human leukocyte antigen (HLA)-A24+ patients with localized prostate cancer received weekly personalized peptide vaccine for six times with positive peptides (up to four kinds of peptides) from 16 kinds of vaccine candidates, followed by a retropubic radical prostatectomy (RRP). Eight patients with localized prostate cancer receiving RRP served as the control group. The serum prostate-specific antigen (PSA) level, and peptide-specific cytotoxic T lymphocyte (CTL) precursor analysis by interferon-γ production, and peptide-reactive immunoglobulin G (IgG) using an enzyme-linked immunosorbent assay were monitored during the treatment. Distributions of CD45RO+ cells, CD8+ T cells, and CD20+ B cells in tissue microarray samples were studied using an immunohistochemical technique.
The peptide vaccination was safe and well tolerated with no major adverse effects. Increased CTL response and the anti-peptide IgG titer were observed in the post-vaccination samples in 8 of 10 or 8 of 10 patients, respectively. The intensity of CD45RO+ infiltrating cells in the vaccination group was significantly larger than that in the control group. CD8+ T cell infiltration was seen only in the vaccinated group.
Increased immune responses, at both the circulation and tumor sites in the vaccinated group, support the further development of personalized peptide vaccines for patients with localized prostate cancer. Prostate 67: 933–942, 2007. © 2007 Wiley-Liss, Inc.