Original Article

Counting alleles in single lesions of prostate tumors from ethnically diverse patients

  1. Aki Morikawa1,2,3,
  2. Vijay Varma2,
  3. Theresa W. Gillespie1,2,
  4. Robert H. Lyles3,
  5. Michael Goodman3,
  6. Roberd M. Bostick1,3,
  7. Jack S. Mandel1,3,
  8. Wei Zhou1,*

Article first published online: 18 DEC 2007

DOI: 10.1002/pros.20693

Issue

The Prostate

The Prostate

Volume 68, Issue 3, pages 231–240, 15 February 2008

Additional Information

How to Cite

Morikawa, A., Varma, V., W. Gillespie, T., Lyles, R. H., Goodman, M., Bostick, R. M., Mandel, J. S. and Zhou, W. (2008), Counting alleles in single lesions of prostate tumors from ethnically diverse patients. The Prostate, 68: 231–240. doi: 10.1002/pros.20693

Author Information

  1. 1

    Emory University School of Medicine, Winship Cancer Institute, Atlanta, Georgia

  2. 2

    Atlanta VA Medical Center, Atlanta, Georgia

  3. 3

    Emory University Rollins School of Public Health, Atlanta, Georgia

*The Winship Cancer Institute, Emory University School of Medicine, Building C, Room 4084, 1365 Clifton Road, NE, Atlanta, GA 30322.

Publication History

  1. Issue published online: 11 JAN 2008
  2. Article first published online: 18 DEC 2007
  3. Manuscript Accepted: 2 OCT 2007
  4. Manuscript Received: 27 JUL 2007

Funded by

  • Department of Defense. Grant Number: PC040315
  • Georgia Cancer Coalition

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Keywords:

  • prostate cancer recurrence;
  • allelic imbalance;
  • surgical margin

Abstract

BACKGROUND

The presence of racial disparities in incidence and mortality rates are well-documented for prostate cancer. Nevertheless, it is unclear whether such disparities are due to genetic alterations that are involved in prostate cancer initiation. Here, we evaluated chromosome 8p allelic loss in a racially diverse cohort.

METHODS

Laser-capture microdissection was used to isolate tumors cells from individual lesions in 153 prostate cancer patients, and 8p allelic status was determined by “counting alleles.” Statistical analyses examined the association between pathologic predictors and biochemical recurrence.

RESULTS AND CONCLUSIONS

Thirty percent of prostate lesions were missing an 8p allele at tumor initiation, while 51% of lesions lost an 8p allele during tumor progression. Biochemical recurrence after radical prostatectomy could be reliably predicted by surgical margin status only in lesions with extensive 8p allelic loss. There was, however, no racial disparity in 8p allelic loss at tumor initiation or during tumor progression, suggesting that the molecular event involved was similar between Caucasians and Africa Americans (CA and AA). Nonetheless, racial differences were present in values of prognostic factors for recurrence. Gleason score was the most important predictor of recurrence (HR = 3.1, 95% CI = 1.1, 9.2) in AA, while among CA, pathologic stage (HR = 3.3, 95% CI = 1.5, 7.6) and surgical margin (HR = 4.7, 95% CI = 1.8, 12.6) were the most important. Therefore, racial disparity in prostate cancer may be due to other factors that are involved in prostate cancer development. Prostate 68: 231–240, 2008. © 2007 Wiley-Liss, Inc.

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