A functional polymorphism in MSMB gene promoter is associated with prostate cancer risk and serum MSMB expression

Authors

  • Bin Xu,

    1. Department of Urology, First Affiliated Hospital of Nanjing Medical University, 300 Guangzhou Road, Nanjing 210029, China
    2. Department of Molecular and Genetic Toxicology, Cancer Center of Nanjing Medical University, 140 Hanzhong Road, Nanjing 210029, China
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  • JinFeng Wang,

    1. Department of Urology, Affiliated Yancheng Hospital, Medical College of Southeast University, Yancheng, China
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  • Na Tong,

    1. Department of Molecular and Genetic Toxicology, Cancer Center of Nanjing Medical University, 140 Hanzhong Road, Nanjing 210029, China
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  • YuanYuan Mi,

    1. Department of Urology, First Affiliated Hospital of Nanjing Medical University, 300 Guangzhou Road, Nanjing 210029, China
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  • ZhiChao Min,

    1. Department of Urology, First Affiliated Hospital of Nanjing Medical University, 300 Guangzhou Road, Nanjing 210029, China
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  • Jun Tao,

    1. Department of Urology, First Affiliated Hospital of Nanjing Medical University, 300 Guangzhou Road, Nanjing 210029, China
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  • PengChao Li,

    1. Department of Urology, First Affiliated Hospital of Nanjing Medical University, 300 Guangzhou Road, Nanjing 210029, China
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  • Gong Cheng,

    1. Department of Urology, First Affiliated Hospital of Nanjing Medical University, 300 Guangzhou Road, Nanjing 210029, China
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  • JiuMing Li,

    1. Department of Urology, BenQ Affiliated Hospital of Nanjing Medical University, Nanjing, China
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  • MeiLin Wang,

    1. Department of Molecular and Genetic Toxicology, Cancer Center of Nanjing Medical University, 140 Hanzhong Road, Nanjing 210029, China
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  • JiaLin Tang,

    1. Department of Molecular and Genetic Toxicology, Cancer Center of Nanjing Medical University, 140 Hanzhong Road, Nanjing 210029, China
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  • NingHong Song,

    1. Department of Urology, First Affiliated Hospital of Nanjing Medical University, 300 Guangzhou Road, Nanjing 210029, China
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  • ZhengDong Zhang,

    1. Department of Molecular and Genetic Toxicology, Cancer Center of Nanjing Medical University, 140 Hanzhong Road, Nanjing 210029, China
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  • Wei Zhang,

    1. Department of Urology, First Affiliated Hospital of Nanjing Medical University, 300 Guangzhou Road, Nanjing 210029, China
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  • HongFei Wu,

    1. Department of Urology, BenQ Affiliated Hospital of Nanjing Medical University, Nanjing, China
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  • LiXin Hua,

    Corresponding author
    1. Department of Urology, First Affiliated Hospital of Nanjing Medical University, 300 Guangzhou Road, Nanjing 210029, China
    • Department of Urology, First Affiliated Hospital of Nanjing Medical University, 300 Guangzhou Road, Nanjing, China.
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  • NingHan Feng

    Corresponding author
    1. Department of Urology, First Affiliated Hospital of Nanjing Medical University, 300 Guangzhou Road, Nanjing 210029, China
    • Department of Urology, First Affiliated Hospital of Nanjing Medical University, 300 Guangzhou Road, Nanjing, China.
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  • Bin Xu, JinFeng Wang, and Na Tong contributed equally to this work.

Abstract

BACKGROUND

To explore the reported association of SNP marker rs10993994 with prostate cancer identified by two independent in two genome-wide association studies (GWAS) further, we performed a case–control study in southern Chinese Han population. Consequently, we detected the serum levels of MSMB expression with different genotypes in the cases and controls to characterize the functional consequences of rs10993994.

MATERIALS AND METHODS

Two hundred fifty-one prostate cancer and 258 control subjects were included in the cancer association study and 90 serum samples were used to test the expression of the MSMB by Enzyme-linked immunosorbent assay (ELISA).

RESULTS

We found that the T allele displayed an increased prevalence of prostate cancer compared with the C allele (OR = 1.30, 95% CI = 1.01–1.67, P = 0.040). Moreover, the prostate cancer patients carrying CT/TT genotype had significantly decreased serum MSMB levels compared to those with CC genotype (16.32 ± 3.98 µg/L vs. 19.33 ± 4.27 µg/L, P = 0.022).

CONCLUSIONS

rs10993994 in MSMB promoter affects serum MSMB expression, contributes to the genetic predisposition to prostate cancer in southern Chinese Han population. Prostate 70: 1146–1152, 2010. © 2010 Wiley-Liss, Inc.

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