The authors have no conflict of interest to disclose.
miR-141 modulates androgen receptor transcriptional activity in human prostate cancer cells through targeting the small heterodimer partner protein†
Article first published online: 7 FEB 2012
Copyright © 2012 Wiley Periodicals, Inc.
Volume 72, Issue 14, pages 1514–1522, 1 October 2012
How to Cite
Xiao, J., Gong, A.-Y., Eischeid, A. N., Chen, D., Deng, C., Young, C. Y.F. and Chen, X.-M. (2012), miR-141 modulates androgen receptor transcriptional activity in human prostate cancer cells through targeting the small heterodimer partner protein. Prostate, 72: 1514–1522. doi: 10.1002/pros.22501
- Issue published online: 24 AUG 2012
- Article first published online: 7 FEB 2012
- Manuscript Accepted: 12 JAN 2012
- Manuscript Received: 23 NOV 2011
- Nebraska Cancer and Smoking-Related Diseases Research Program. Grant Numbers: LB506, LB595
- androgen receptor;
- prostate cancer;
Aberrant expressions of microRNAs, including upregulation of miR-141, are closely associated with the tumorigenesis of prostate cancer (PCa). The orphan receptor small heterodimer partner (Shp) is a co-repressor to androgen receptor (AR) and represses AR-regulated transcriptional activity.
Here, we investigated the correlation of Shp expression with the cellular level of miR-141 and its effects on AR transcriptional activity in non-malignant and malignant human prostate epithelial cell lines.
We found that Shp was downregulated in multiple PCa cell lines. The mature form of miR-141 was upregulated in PCa cells. miR-141 could target 3′-untranslated region of Shp mRNA resulting in translational suppression and RNA degradation. Moreover, enforced expression of Shp or inhibition of miR-141 function by anti-miR-141 attenuated AR-regulated transcriptional activity in AR-responsive LNCaP cells. Phenethyl isothiocyanate, a natural constituent of many edible cruciferous vegetables, increased Shp expression, downregulated miR-141, and inhibited AR transcriptional activity in LNCaP cells.
Shp is a target for miR-141 and it is downregulated in cultured human PCa cells with the involvement of upregulation of miR-141, which promotes AR transcriptional activity. Moreover, Shp and miR-141 could be targets for chemoprevention for PCa. Prostate 72:1514–1522, 2012. © 2012 Wiley Periodicals, Inc.