Disclosure Statement: No conflicts of interest, financial or otherwise, are declared by the author.
Contractile signaling pathways in mouse prostate smooth muscle†
Article first published online: 6 FEB 2013
Copyright © 2013 Wiley Periodicals, Inc.
Volume 73, Issue 9, pages 996–1006, June 2013
How to Cite
Kitazawa, T. (2013), Contractile signaling pathways in mouse prostate smooth muscle. Prostate, 73: 996–1006. doi: 10.1002/pros.22647
- Issue published online: 25 MAY 2013
- Article first published online: 6 FEB 2013
- Manuscript Accepted: 8 JAN 2013
- Manuscript Received: 22 NOV 2012
- muscarinic receptor;
Prostate smooth muscle plays an important role in the physiological ejection of prostatic fluid and also in the pathogenesis of benign prostate hyperplasia. Although mouse is the best genetically engineered animal model to identify potential molecular targets for human diseases, only fragmentary information is available for basic mechanism of mouse prostate contraction.
Small smooth muscle tubular rings were excised from four mouse prostate lobes to measure their isometric contractions. High K+, noradrenaline (NA), or acetylcholine (ACh) was applied with and without various antagonists and/or inhibitors to examine the contractile signaling pathways.
Maximum amplitude of agonist-induced contractions varied greatly with different lobes but not with different locations or orientations within each lobe. Both NA and ACh produced large contractions in ventral and dorsal rings, whereas only small contractions were elicited in lateral and anterior rings. Combination of alpha-1 and muscarinic antagonists suppressed K+ depolarization-induced contraction potently in ventral rings, but slightly in anterior rings. Blocking of either Ca2+-release or Ca2+-influx reduced agonist-induced contraction of ventral rings, however, a considerable amount of contractility remained even with both blockers. Inhibitors of ROCK and PKC partially inhibited NA-induced contractions, whereas a combination of Ca2+-blockers and Ca2+-sensitization inhibitors strongly suppressed the contraction.
The ejection of prostatic fluid is differentially regulated in each prostate lobe. In ventral prostate smooth muscle, Ca2+-release, Ca2+-influx, and ROCK- and PKC-mediated Ca2+-sensitizations are all involved in NA-induced contractions. This finding is a useful step toward the understanding of the phenotypic changes in the smooth muscle of BPH prostate. Prostate 73: 996–1006, 2013. © 2013 Wiley Periodicals, Inc.