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Keywords:

  • calcium;
  • Ca2+;
  • prostate;
  • cancer;
  • clearance

Abstract

BACKGROUND

Three prostatic cell lines, PC3, LNCaP, and DU 145, are used as established models to study cell signaling in prostate cancer. Recently, stromal cell lines of the prostate, such as P21, were also introduced. Here we investigate a basic and important mechanism of living cells: Ca2+ homeostasis in PC3, DU 145, and P21.

METHODS

We examined Ca2+ clearance mechanisms by monitoring the kinetics of recovery from histamine stimulation under conditions which inhibit prospect mechanisms for storing or extrusion of Ca2+ from the cytosol by photometry.

RESULTS

Despite the fact that in all three cell lines the Ca2+ ATPase of the plasma membrane and the SERCA are most important for Ca2+ homeostasis, inhibition of PMCA in epithelial cells has a greater effect than in stromal cells. Furthermore, the proportion of PMCA and SERCA differs in PC3 and DU145 cells. PMCA is most effective at reaching resting [Ca2+]i in the final recovery stage. In contrast to DU 145 and P21 cells, PC3 are the only cells substantially affected by the inhibition of the mitochondrial uniporter. In all cell lines the role of the sodium calcium exchanger is marginal.

CONCLUSION

These results demonstrate that not only cancer and stromal cell lines show significant differences in the modes and extent of their use of Ca2+ clearance mechanisms, but also the cancer cell lines themselves. Prostate 74:29–40, 2014. © 2013 Wiley Periodicals, Inc.