The authors declare no competing interests.
Ca2+ clearance mechanisms in cancer cell lines and stromal cells of the prostate
Version of Record online: 4 SEP 2013
© 2013 Wiley Periodicals, Inc.
Volume 74, Issue 1, pages 29–40, January 2014
How to Cite
Wolf, A. and Wennemuth, G. (2014), Ca2+ clearance mechanisms in cancer cell lines and stromal cells of the prostate. Prostate, 74: 29–40. doi: 10.1002/pros.22724
- Issue online: 6 DEC 2013
- Version of Record online: 4 SEP 2013
- Manuscript Accepted: 8 AUG 2013
- Manuscript Received: 20 JUN 2013
- Departmental Resources
Three prostatic cell lines, PC3, LNCaP, and DU 145, are used as established models to study cell signaling in prostate cancer. Recently, stromal cell lines of the prostate, such as P21, were also introduced. Here we investigate a basic and important mechanism of living cells: Ca2+ homeostasis in PC3, DU 145, and P21.
We examined Ca2+ clearance mechanisms by monitoring the kinetics of recovery from histamine stimulation under conditions which inhibit prospect mechanisms for storing or extrusion of Ca2+ from the cytosol by photometry.
Despite the fact that in all three cell lines the Ca2+ ATPase of the plasma membrane and the SERCA are most important for Ca2+ homeostasis, inhibition of PMCA in epithelial cells has a greater effect than in stromal cells. Furthermore, the proportion of PMCA and SERCA differs in PC3 and DU145 cells. PMCA is most effective at reaching resting [Ca2+]i in the final recovery stage. In contrast to DU 145 and P21 cells, PC3 are the only cells substantially affected by the inhibition of the mitochondrial uniporter. In all cell lines the role of the sodium calcium exchanger is marginal.
These results demonstrate that not only cancer and stromal cell lines show significant differences in the modes and extent of their use of Ca2+ clearance mechanisms, but also the cancer cell lines themselves. Prostate 74:29–40, 2014. © 2013 Wiley Periodicals, Inc.