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Research Article
Fluorescent dyes as probes to study lipid-binding proteins
Article first published online: 12 SEP 2003
DOI: 10.1002/prot.10401
Copyright © 2003 Wiley-Liss, Inc.
Issue
1097-0134/asset/cover.gif?v=1&s=d817e79b67ba6cacf8bdcce1a819c04de300a7e3 "Proteins: Structure, Function, and Bioinformatics")
Proteins: Structure, Function, and Bioinformatics
Volume 53, Issue 3, pages 607–615, 15 November 2003
Additional Information
How to Cite
Pastukhov, A. V. and Ropson, I. J. (2003), Fluorescent dyes as probes to study lipid-binding proteins. Proteins, 53: 607–615. doi: 10.1002/prot.10401
Publication History
- Issue published online: 16 OCT 2003
- Article first published online: 12 SEP 2003
- Manuscript Accepted: 12 DEC 2002
- Manuscript Received: 23 MAY 2002
- Abstract
- Article
- References
- Cited By
Keywords:
- lipid-binding proteins;
- ANS, bisANS;
- binding;
- fluorescence
Abstract
We studied the equilibrium binding of two hydrophobic fluorescent dyes, ANS and bisANS, to four members of a family of intracellular lipid-binding proteins: IFABP, CRABP I, CRABP II, and ILBP. The spectral and binding parameters for the probes bound to the proteins were determined. Typically, there was a single binding site on each protein for the ligands. However, IFABP cooperatively bound a second bisANS molecule in the binding pocket. Comparative analysis of affinities and spectral characteristics for the two probes allowed us to examine the contributions of electrostatic and hydrophobic interactions to the binding process, and to address some aspects of the internal structure of the studied proteins. Proteins 2003;53:000–000. © 2003 Wiley-Liss, Inc.